NDLI: Mechanism of Calcite Crystal Growth Inhibition by the N-terminal Undecapeptide of Lithostathine

Content Provider	Journal of Biological Chemistry (JBC)
Author	Gerbaud, Vincent Pignol, David Loret, Erwann Bertrand, Jay A. Berland, Yvon Fontecilla-Camps, Juan-Carlos Canselier, Jean-Paul Gabas, Nadine Verdier, Jean-Michel
Abstract	Pancreatic juice is supersaturated with calcium carbonate. Calcite crystals therefore may occur, obstruct pancreatic ducts, and finally cause a lithiasis. Human lithostathine, a protein synthesized by the pancreas, inhibits the growth of calcite crystals by inducing a habit modification: the rhombohedral {10 1̄4} usual habit is transformed into a needle-like habit through the {112̄0} crystal form. A similar observation was made with the N-terminal undecapeptide (pE1R11) of lithostathine. We therefore aimed at discovering how peptides inhibit calcium salt crystal growth. We solved the complete x-ray structure of lithostathine, including the flexible N-terminal domain, at 1.3 Å. Docking studies of pE1R11 with the (101̄4) and (11 2̄0) faces through molecular dynamics simulation resulted in three successive steps. First, the undecapeptide progressively unfolded as it approached the calcite surface. Second, mobile lateral chains of amino acids made hydrogen bonds with the calcite surface. Last, electrostatic bonds between calcium ions and peptide bonds stabilized and anchored pE1R11 on the crystal surface. pE1R11-calcite interaction was stronger with the (11 2̄0) face than with the (10 1̄4) face, confirming earlier experimental observations. Energy contributions showed that the peptide backbone governed the binding more than did the lateral chains. The ability of peptides to inhibit crystal growth is therefore essentially based on backbone flexibility.
Related Links	http://www.jbc.org/content/275/2/1057.abstract
Ending Page	1064
Starting Page	1057
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	2
Volume Number	275
DOI	10.1074/jbc.275.2.1057
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-01-14
Access Restriction	Open
Subject Keyword	N-(9-fluorenyl)methoxycarbonyl (Fmoc) High pressure liquid chromatography (HPLC) Pyroglutamic acid (pyro-Glu) PROTEIN CHEMISTRY AND STRUCTURE
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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