NDLI: Rho GTPase Control of Protein Kinase C-related Protein Kinase Activation by 3-Phosphoinositide-dependent Protein Kinase

Content Provider	Journal of Biological Chemistry (JBC)
Author	Flynn, Peter Mellor, Harry Casamassima, Adele Parker, Peter J.
Abstract	The protein kinase C-related protein kinases (PRKs) have been shown to be under the control of the Rho GTPases and influenced by autophosphorylation. In analyzing the relationship between these inputs, it is shown that activation in vitroand in vivo involves the activation loop phosphorylation of PRK1/2 by 3-phosphoinositide-dependent protein kinase-1 (PDK1). Rho overexpression in cultured cells is shown to increase the activation loop phosphorylation of endogenous PRKs and is demonstrated to influence this process by controlling the ability of PRKs to bind to PDK1. The interaction of PRK1/2 with PDK1 is shown to be dependent upon Rho. Direct demonstration of ternary (Rho·PRK·PDK1) complex formation in situ is provided by the observation that PDK1 is recruited to RhoB-containing endosomes only if PRK is coexpressed. Furthermore, this in vivo complex is maintained after phosphoinositide 3-kinase inhibition. The control of PRKs by PDK1 thus evidences a novel strategy of substrate-directed control involving GTPases.
Related Links	http://www.jbc.org/content/275/15/11064.abstract
Ending Page	11070
Starting Page	11064
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	15
Volume Number	275
DOI	10.1074/jbc.275.15.11064
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2000-04-14
Access Restriction	Open
Subject Keyword	Protein kinase C-related protein kinases (PRKs) Protein kinase C (PKC) Guanosine 5′-O-(3-thiotriphosphate) (GTPγS) 3-phosphoinositide-dependent protein kinase (PDK) Protein kinase B (PKB) Phosphatidylinositol (PtdIns) Pleckstrin homology (PH) Phosphoinositide 3-kinase (PI3K) Glutathione S-transferase (GST) Dithiothreitol (DTT) Myelin basic protein (MBP) MECHANISMS OF SIGNAL TRANSDUCTION
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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