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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Zhu, Xiaoling Bansal, Niharika P. Evans, Janice P. |
| Abstract | Fertilin β (also known as ADAM2) is a cell adhesion molecule on the surface of mammalian sperm that participates in sperm-egg membrane binding. Fertilin β is a member of the molecular family known as ADAMs or MDCs. These proteins have a disintegrin domain with homology to integrin ligands found in snake venoms; several of these snake proteins have an RGD tripeptide presented on an extended “disintegrin loop.” However, fertilin β lacks an RGD tripeptide and instead has the consensus sequenceX(D/E)ECD (QDECD in mouse fertilin β) in its putative disintegrin loop, and there is controversy over which amino acids comprise the active site of the fertilin β disintegrin loop. We have used point-mutated versions of the sequence AQDECDVT and two bioassays to identify the key functional amino acids of this sequence from the mouse fertilin β disintegrin domain. Amino acid substitutions for the terminal aspartic acid residue of the QDECD sequence result in dramatically reduced activities in the two assays for protein function, implicating the terminal aspartic acid residue as critical for protein function. Substitutions for the glutamic acid and the cysteine residues in the QDECD sequence result in slight reductions in activity, whereas substitution of the first aspartic acid has virtually no effect. These data suggest that the conserved ECD sequence of the mouse fertilin β disintegrin loop, especially the terminal D residue, contributes more to the protein's activity than does the QDE sequence that aligns with the RGD tripeptide in other disintegrins. |
| Related Links | http://www.jbc.org/content/275/11/7677.abstract |
| Ending Page | 7683 |
| Starting Page | 7677 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 11 |
| Volume Number | 275 |
| DOI | 10.1074/jbc.275.11.7677 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2000-03-17 |
| Access Restriction | Open |
| Subject Keyword | Disintegrin loop (DL) Fertilin β DL (βDL) Fertilin β disintegrin/cysteine-rich/epidermal growth factor-like repeat (βDCE) Maltose-binding protein (MBP) Bacterial alkaline phosphatase (BAP) Zona pellucida (ZP) Fertilin β disintegrin domain (βD) MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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