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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Hishikawa, Keiichi Oemar, Barry S. Tanner, Felix C. Nakaki, Toshio Lüscher, Thomas F. Fujii, Tomoko |
| Abstract | Connective tissue growth factor (CTGF) is a member of an emerging CCN gene family that is implicated in various diseases associated with fibro-proliferative disorder including scleroderma and atherosclerosis. The function of CTGF in human cancer is largely unknown. We now show that CTGF induces apoptosis in the human breast cancer cell line MCF-7. CTGF mRNA was completely absent in MCF-7 but strongly induced by treatment with transforming growth factor β (TGF-β). TGF-β by itself induced apoptosis in MCF-7, and this effect was reversed by co-treatment with CTGF antisense oligonucleotide. Overexpression of CTGF gene in transiently transfected MCF-7 cells significantly augmented apoptosis. Moreover, recombinant CTGF protein significantly enhanced apoptosis in MCF-7 cells as evaluated by DNA fragmentation, Tdt-mediated dUTP biotin nick end-labeling staining, flow cytometry analysis, and nuclear staining using Hoechst 33258. Finally, recombinant CTGF showed no effect on Bax protein expression but significantly reduced Bcl2 protein expression. Taken together, these results suggest that CTGF is a major inducer of apoptosis in the human breast cancer cell line MCF-7 and that TGF-β-induced apoptosis in MCF-7 cells is mediated, in part, by CTGF. |
| Related Links | http://www.jbc.org/content/274/52/37461.abstract |
| Ending Page | 37466 |
| Starting Page | 37461 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 52 |
| Volume Number | 274 |
| DOI | 10.1074/jbc.274.52.37461 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 1999-12-24 |
| Access Restriction | Open |
| Subject Keyword | Connective tissue growth factor, CMV, cytomegalo virus, TGF-β, transforming growth factor β (CTGF) Tdt-mediated dUTP biotin nick end-labeling (TUNEL) Dulbecco's modified Eagle's medium (DMEM) CELL BIOLOGY AND METABOLISM |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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