NDLI: The Src Homology Domain 2-Containing Inositol Phosphatase SHIP Forms a Ternary Complex with Shc and Grb2 in Antigen Receptor-stimulated B Lymphocytes

Content Provider	Journal of Biological Chemistry (JBC)
Author	Harmer, Stacey L. DeFranco, Anthony L.
Abstract	The inositol phosphatase SHIP has been implicated in signaling events downstream of a variety of receptors and is thought to play an inhibitory role in stimulated B cells. We and others have reported that SHIP is rapidly tyrosine phosphorylated upon B cell antigen receptor (BCR) cross-linking and forms a complex with the adapter protein Shc. Here, we report that cross-linking of the BCR induces association between Grb2 and SHIP as well as association between Shc and SHIP. We made use of a Grb2-deficient B cell line to demonstrate both in vitro and in vivo that Grb2 expression is required for the efficient association between Shc and SHIP. The results indicate that SHIP, Shc, and Grb2 form a ternary complex in stimulated B cells, with Grb2 stabilizing the interaction between Shc and SHIP. The interactions between Shc, Grb2, and SHIP are therefore analogous to the interactions between Shc, Grb2, and SOS. Shc and Grb2 may help to localize SHIP to the cell membrane, regulating SHIP’s inhibitory function following BCR stimulation.
Related Links	http://www.jbc.org/content/274/17/12183.abstract
Ending Page	12191
Starting Page	12183
Page Count	9
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	17
Volume Number	274
DOI	10.1074/jbc.274.17.12183
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1999-04-23
Access Restriction	Open
Subject Keyword	B cell antigen receptor (BCR) Phosphatidylinositol 3-kinase (PI 3-kinase) SH2-containing inositol phosphatase (SHIP) 4)P2, phosphatidylinositol 3,4-bisphosphate (PI(3) Src homology domain 2 (SH2) Glutathione S-transferase (GST) Polyacrylamide gel electrophoresis (PAGE) Hemagglutinin (HA) Mitogen-activated protein kinase (MAPK) CELL BIOLOGY AND METABOLISM
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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