NDLI: The Pre-transmembrane 1 Domain of Acid-sensing Ion Channels Participates in the Ion Pore

Content Provider	Journal of Biological Chemistry (JBC)
Author	Coscoy, Sylvie Weille, Jan R. De Lingueglia, Eric Lazdunski, Michel
Abstract	The acid-sensing ion channel (ASIC) subunits ASIC1, ASIC2, and ASIC3 are members of the amiloride-sensitive Na+ channel/degenerin family of ion channels. They form proton-gated channels that are expressed in the central nervous system and in sensory neurons, where they are thought to play an important role in pain accompanying tissue acidosis. A splice variant of ASIC2, ASIC2b, is not active on its own but modifies the properties of ASIC3. In particular, whereas most members of the amiloride-sensitive Na+ channel/degenerin family are highly selective for Na+ over K+, ASIC3/ASIC2b heteromultimers show a nonselective component. Chimeras of the two splice variants allowed identification of a 9-amino acid region preceding the first transmembrane (TM) domain (pre-TM1) of ASIC2 that is involved in ion permeation and is critical for Na+ selectivity. Three amino acids in this region (Ile-19, Phe-20, and Thr-25) appear to be particularly important, because channels mutated at these residues discriminate poorly between Na+ and K+. In addition, the pH dependences of the activity of the F20S and T25K mutants are changed as compared with that of wild-type ASIC2. A corresponding ASIC3 mutant (T26K) also has modified Na+selectivity. Our results suggest that the pre-TM1 region of ASICs participates in the ion pore.
Related Links	http://www.jbc.org/content/274/15/10129.abstract
Ending Page	10132
Starting Page	10129
Page Count	4
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	15
Volume Number	274
DOI	10.1074/jbc.274.15.10129
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1999-04-09
Access Restriction	Open
Subject Keyword	Acid-sensing ion channel (ASIC) Epithelial Na+ channel (ENaC) Phe-Met-Arg-Phe-amide-gated channel (FaNaC) Polymerase chain reaction (PCR) Transmembrane (TM) Amiloride-sensitive Na+channel/degenerin (NaC/DEG) MEMBRANES AND BIOENERGETICS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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