NDLI: Specific Cu2+-catalyzed Oxidative Cleavage of Na,K-ATPase at the Extracellular Surface

Content Provider	Journal of Biological Chemistry (JBC)
Author	Shimon, Meirav Bar Goldshleger, Rivka Karlish, Steven J. D.
Abstract	This paper describes specific Cu2+-catalyzed oxidative cleavage of α and β subunits of Na,K-ATPase at the extracellular surface. Incubation of right side-out renal microsomal vesicles with Cu2+ ions, ascorbate, and H2O2 produces two major cleavages of the α subunit within the extracellular loop between trans-membrane segments M7 and M8 and L7/8. Minor cleavages are also detected in loops L9/10 and L5/6. In the β subunit two cleavages are detected, one before the first S-S bridge and the other between the second and third S-S bridges. Na,K-ATPase and Rb+ occlusion are inactivated after incubation with Cu2+/ascorbate/H2O2. These observations are suggestive of a site-specific mechanism involving cleavage of peptide bonds close to a bound Cu2+ ion. This mechanism allows several inferences on subunit interactions and spatial organization. The two cleavage sites in L7/8 of the α subunit and two cleavage sites of the β subunit identify interacting segments of the subunits. L7/8 is also close to L9/10 and to cation occlusion sites. Comparison of the locations of Cu2+-catalyzed cleavages with Fe2+-catalyzed cleavages (Goldshleger, R., and Karlish, S. J. D. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 9596–9601) suggests division of the membrane sector into two domains comprising M1–M6 and M7–M10/Mβ, respectively.
Related Links	http://www.jbc.org/content/273/51/34190.abstract
Ending Page	34195
Starting Page	34190
Page Count	6
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	51
Volume Number	273
DOI	10.1074/jbc.273.51.34190
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1998-12-18
Access Restriction	Open
Subject Keyword	Hydrogen peroxide, PNGase, peptide-N-glycosidase F (H2O2) N-[tris(hydroxymethyl]glycine (Tricine) Polyvinylidene difluoride (PVDF) Polyacrylamide gel electrophoresis. (PAGE) MEMBRANES AND BIOENERGETICS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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