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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Brodie, Chaya Bogi, Krisztina Acs, Peter Lorenzo, Patricia S. Baskin, Lindsey Blumberg, Peter M. |
| Abstract | Protein kinase C (PKC) plays an important role in the proliferation and differentiation of glial cells. In a recent study we found that overexpression of PKCδ reduced the expression of the astrocytic marker glutamine synthetase (GS). In this study we explored the mechanisms involved in the inhibitory effect of PKCδ on the expression of glutamine synthetase. Using PKC chimeras we first examined the role of the catalytic and regulatory domains of PKCδ on the expression of glutamine synthetase. We found that cells stably transfected with chimeras between the regulatory domain of PKCδ and the catalytic domains of PKCα or ε inhibited the expression of GS, similar to the inhibition exerted by overexpression of PKCδ itself. In contrast, no significant effects were observed in cells transfected with the reciprocal PKC chimeras between the regulatory domains of PKCα or ε and the catalytic domain of PKCδ. PKCδ has been shown to undergo tyrosine phosphorylation in response to various activators. Tyrosine phosphorylation of PKCδ in response to phorbol 12-myristate 13-acetate and platelet-derived growth factor occurred only in chimeras which contained the PKCδ regulatory domain. Cells transfected with a PKCδ mutant (PKCδ5), in which the five putative tyrosine phosphorylation sites were mutated to phenylalanine, showed markedly diminished tyrosine phosphorylation in response to phorbol 12-myristate 13-acetate and platelet-derived growth factor and normal levels of GS. Our results indicate that the regulatory domain of PKCδ mediates the inhibitory effect of this isoform on the expression of GS. Phosphorylation of PKCδ on tyrosine residues in the regulatory domain is implicated in this inhibitory effect. |
| Related Links | http://www.jbc.org/content/273/46/30713.abstract |
| Ending Page | 30718 |
| Starting Page | 30713 |
| Page Count | 6 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 46 |
| Volume Number | 273 |
| DOI | 10.1074/jbc.273.46.30713 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 1998-11-13 |
| Access Restriction | Open |
| Subject Keyword | Protein kinase C (PKC) Phorbol 12-myristate 13-acetate (PMA) Glial fibrillary acidic protein (GFAP) Glutamine synthetase (GS) Polyacrylamide gel electrophoresis (PAGE) Platelet-derived growth factor (PDGF) Bryostatin 1. (Bryo) CELL BIOLOGY AND METABOLISM |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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