NDLI: Biosynthesis of Branched Polylactosaminoglycans EMBRYONAL CARCINOMA CELLS EXPRESS MIDCHAIN β1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE ACTIVITY THAT GENERATES BRANCHES TO PREFORMED LINEAR BACKBONES

Content Provider	Journal of Biological Chemistry (JBC)
Author	Leppänen, Anne Zhu, Ying Maaheimo, Hannu Helin, Jari Lehtonen, Eero Renkonen, Ossi
Abstract	Two types of β1,6-GlcNAc transferases (IGnT6) are involved in in vitro branching of polylactosamines: dIGnT6 (distally acting), transferring to the penultimate galactose residue in acceptors like GlcNAcβ1–3Galβ1–4GlcNAcβ1-R, and cIGnT6 (centrally acting), transferring to the midchain galactoses in acceptors of the type (GlcNAcβ1–3)Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAcβ1-R. The roles of the two transferases in the biosynthesis of branched polylactosamine backbones have not been clearly elucidated. We report here that cIGnT6 activity is expressed in human (PA1) and murine (PC13) embryonal carcinoma (EC) cells, both of which contain branched polylactosamines in large amounts. In the presence of exogenous UDP-GlcNAc, lysates from both EC cells catalyzed the formation of the branched pentasaccharide Galβ1–4GlcNAcβ1–3(GlcNAcβ1–6)Galβ1–4GlcNAc from the linear tetrasaccharide Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAc. The PA1 cell lysates were shown to also catalyze the formation of the branched heptasaccharides Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAcβ1–3(GlcNAcβ1–6)Galβ1–4GlcNAc and Galβ1–4GlcNAcβ1–3(GlcNAcβ1–6)Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAc from the linear hexasaccharide Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAc in reactions characteristic to cIGnT6. By contrast, dIGnT6 activity was not detected in the lysates of the two EC cells that were incubated with UDP-GlcNAc and the acceptor trisaccharide GlcNAcβ1–3Galβ1–4GlcNAc. Hence, it appears likely that cIGnT6, rather than dIGnT6 is responsible for the synthesis of the branched polylactosamine chains in these cells.
Related Links	http://www.jbc.org/content/273/28/17399.abstract
Ending Page	17405
Starting Page	17399
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	28
Volume Number	273
DOI	10.1074/jbc.273.28.17399
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1998-07-10
Access Restriction	Open
Subject Keyword	CARBOHYDRATES, LIPIDS, AND OTHER NATURAL PRODUCTS
Alternative Title	Biosynthesis of Branched Polylactosaminoglycans
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Gain-of-function Chinese Hamster Ovary Mutants LEC18 and LEC14 Each Express a Novel N-Acetylglucosaminyltransferase Activity

Altered Golgi Localization of Core 2 β-1,6-N-Acetylglucosaminyltransferase Leads to Decreased Synthesis of Branched O-Glycans

Transcriptional Regulation of the N-Acetylglucosaminyltransferase V Gene in Human Bile Duct Carcinoma Cells (HuCC-T1) Is Mediated by Ets-1

Purification and Characterization of UDP-N-Acetylglucosamine: α1,3-d-Mannoside β1,4-N-Acetylglucosaminyltransferase (N-Acetylglucosaminyltransferase-IV) from Bovine Small Intestine

Tissue-specific Regulation of Mouse Core 2 β-1,6-N-Acetylglucosaminyltransferase

Circular Dichroic Spectroscopy of N-Acetylglucosaminyltransferase V and Its Substrate Interactions

Regulation of I-Branched Poly-N-Acetyllactosamine Synthesis CONCERTED ACTIONS BY i-EXTENSION ENZYME, I-BRANCHING ENZYME, AND β1,4-GALACTOSYLTRANSFERASE I

Transforming Growth Factor β Up-regulates Expression of the N-Acetylglucosaminyltransferase V Gene in Mouse Melanoma Cells

cDNA Cloning and Expression of Bovine UDP-N-Acetylglucosamine:α1,3-d-Mannoside β1,4-N-Acetylglucosaminyltransferase IV

Biosynthesis of Branched Polylactosaminoglycans EMBRYONAL CARCINOMA CELLS EXPRESS MIDCHAIN β1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE ACTIVITY THAT GENERATES BRANCHES TO PREFORMED LINEAR BACKBONES

Similar Documents

Gain-of-function Chinese Hamster Ovary Mutants LEC18 and LEC14 Each Express a Novel N-Acetylglucosaminyltransferase Activity

Altered Golgi Localization of Core 2 β-1,6-N-Acetylglucosaminyltransferase Leads to Decreased Synthesis of Branched O-Glycans

Transcriptional Regulation of the N-Acetylglucosaminyltransferase V Gene in Human Bile Duct Carcinoma Cells (HuCC-T1) Is Mediated by Ets-1

Purification and Characterization of UDP-N-Acetylglucosamine: α1,3-d-Mannoside β1,4-N-Acetylglucosaminyltransferase (N-Acetylglucosaminyltransferase-IV) from Bovine Small Intestine

Tissue-specific Regulation of Mouse Core 2 β-1,6-N-Acetylglucosaminyltransferase

Circular Dichroic Spectroscopy of N-Acetylglucosaminyltransferase V and Its Substrate Interactions

Regulation of I-Branched Poly-N-Acetyllactosamine Synthesis CONCERTED ACTIONS BY i-EXTENSION ENZYME, I-BRANCHING ENZYME, AND β1,4-GALACTOSYLTRANSFERASE I

Transforming Growth Factor β Up-regulates Expression of the N-Acetylglucosaminyltransferase V Gene in Mouse Melanoma Cells

cDNA Cloning and Expression of Bovine UDP-N-Acetylglucosamine:α1,3-d-Mannoside β1,4-N-Acetylglucosaminyltransferase IV

Biosynthesis of Branched Polylactosaminoglycans EMBRYONAL CARCINOMA CELLS EXPRESS MIDCHAIN β1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE ACTIVITY THAT GENERATES BRANCHES TO PREFORMED LINEAR BACKBONES