NDLI: A Cell Binding Domain from the α3 Chain of Type IV Collagen Inhibits Proliferation of Melanoma Cells

Content Provider	Journal of Biological Chemistry (JBC)
Author	Han, Jing Ohno, Nobuko Pasco, Sylvie Monboisse, Jean-Claude Borel, Jacques P. Kefalides, Nicholas A.
Abstract	Our previous studies have shown that a peptide corresponding to the residue sequence 185–203 of the NC1 domain of the α3 chain of basement membrane collagen (type IV) inhibits the activation of polymorphonuclear leukocytes. Peptides from the same region of the α1, α2, α4, and α5(IV) chains did not exhibit this property. Because of the intimate relationship between metastasizing neoplastic cells and vascular as well as epithelial basement membranes, we measured the cell adhesion-promoting activity of peptides from the NC1 domain of type IV collagen and their effect on proliferation of human melanoma cells. We found that peptide α3(IV)185–203 (CNYYSNSYSFWLASLNPER) not only promotes adhesion of human melanoma cells but also inhibits their proliferation. Adhesion increased by 50–60% over control. Melanoma cell proliferation was inhibited by 40% when cells were grown in a medium containing 5 μg/ml peptide for 5 days. Studies showed that replacement of serine in position 189 or 191 by alanine resulted in significantly reduced adhesion. Similarly, serine replacement resulted in reduced ability to inhibit proliferation. Our data suggest that a region of the NC1 domain of the α3(IV) chain, contained within the sequence 185–203, not only specifically promotes adhesion but also inhibits proliferation of melanoma cells. These properties appear to be dependent on the presence of the triplet sequence -SNS- (residues 189–191), which is unique to the α3 chain and may represent an important functional epitope.
Related Links	http://www.jbc.org/content/272/33/20395.abstract
Ending Page	20401
Starting Page	20395
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	33
Volume Number	272
DOI	10.1074/jbc.272.33.20395
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1997-08-15
Access Restriction	Open
Subject Keyword	CELL BIOLOGY AND METABOLISM
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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