NDLI: NF-κB-mediated Induction of mdr1b Expression by Insulin in Rat Hepatoma Cells

Content Provider	Journal of Biological Chemistry (JBC)
Author	Zhou, Ge Kuo, M. Tien
Abstract	The expression of P-glycoproteins encoded by themdr gene family is associated with the emergence of multidrug resistance phenotype in animal cells. However, the mechanisms controlling the expression of these genes have not been well elucidated. Here, we report that the expression of ratmdr1b gene in cultured H-4-II-E hepatoma cells can be induced by insulin. Transient transfection assays using reporter gene constructs containing various 5′ mdr1b sequences showed that the sequence located between base pairs −243 and −163 is important for insulin’s induction of mdr1b promoter activity. Further analyses revealed that a NF-κB-binding site (located between base pairs −167 and −158) is required for insulin-induced promoter activity. Gel mobility shift assay demonstrated that insulin stimulates the binding of nuclear p50/p65 subunits to the mdr1b NF-κB sequence. Cotransfection of plasmids expressing either the p50/p65 NF-κB subunits or Raf-1 kinase or both resulted in increased expression of the gene containing wild-type but not NF-κB site-mutated mdr1b promoter. Finally, expression of either the antisense p65 subunit of NF-κB or dominant negative Raf-1 kinase blocked insulin’s induction of themdr1b promoter activity. Taken together, our results suggest that the insulin-induced mdr1b expression is mediated by transcription factor NF-κB via the Raf-1 kinase signaling pathway.
Related Links	http://www.jbc.org/content/272/24/15174.abstract
Ending Page	15183
Starting Page	15174
Page Count	10
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	24
Volume Number	272
DOI	10.1074/jbc.272.24.15174
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1997-06-13
Access Restriction	Open
Subject Keyword	NUCLEIC ACIDS, PROTEIN SYNTHESIS, AND MOLECULAR GENETICS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Elevated Cyclic AMP Inhibits NF-κB-mediated Transcription in Human Monocytic Cells and Endothelial Cells

Induction and Stabilization of IκBα by Nitric Oxide Mediates Inhibition of NF-κB

Inhibition of Gene Expression by Triple Helix Formation in Hepatoma Cells

Identification and Characterization of a Hepatoma Cell-specific Enhancer in the Mouse Multidrug Resistance mdr1b Promoter

Mechanism of Sodium Arsenite-mediated Induction of Heme Oxygenase-1 in Hepatoma Cells ROLE OF MITOGEN-ACTIVATED PROTEIN KINASES

Concerted Participation of NF-κB and C/EBP Heteromer in Lipopolysaccharide Induction of Serum Amyloid A Gene Expression in Liver

Cell Type- and Developmental Stage-specific Activation of NF-κB by fMet-Leu-Phe in Myeloid Cells

Platelet-derived Growth Factor Induction of the Immediate-early gene MCP-1 Is Mediated by NF-κB and a 90-kDa Phosphoprotein Coactivator

Transcriptional Induction of Cholesterol 7α-Hydroxylase by Dexamethasone in L35 Hepatoma Cells Requires Sulfhydryl Reducing Agents

NF-κB-mediated Induction of mdr1b Expression by Insulin in Rat Hepatoma Cells

Similar Documents

Elevated Cyclic AMP Inhibits NF-κB-mediated Transcription in Human Monocytic Cells and Endothelial Cells

Induction and Stabilization of IκBα by Nitric Oxide Mediates Inhibition of NF-κB

Inhibition of Gene Expression by Triple Helix Formation in Hepatoma Cells

Identification and Characterization of a Hepatoma Cell-specific Enhancer in the Mouse Multidrug Resistance mdr1b Promoter

Mechanism of Sodium Arsenite-mediated Induction of Heme Oxygenase-1 in Hepatoma Cells ROLE OF MITOGEN-ACTIVATED PROTEIN KINASES

Concerted Participation of NF-κB and C/EBP Heteromer in Lipopolysaccharide Induction of Serum Amyloid A Gene Expression in Liver

Cell Type- and Developmental Stage-specific Activation of NF-κB by fMet-Leu-Phe in Myeloid Cells

Platelet-derived Growth Factor Induction of the Immediate-early gene MCP-1 Is Mediated by NF-κB and a 90-kDa Phosphoprotein Coactivator

Transcriptional Induction of Cholesterol 7α-Hydroxylase by Dexamethasone in L35 Hepatoma Cells Requires Sulfhydryl Reducing Agents

NF-κB-mediated Induction of mdr1b Expression by Insulin in Rat Hepatoma Cells