NDLI: cDNA Cloning and Expression of a Novel Human UDP-N-acetyl-α-D-galactosamine POLYPEPTIDE N-ACETYLGALACTOSAMINYLTRANSFERASE, GalNAc-T3

Content Provider	Journal of Biological Chemistry (JBC)
Author	Bennett, Eric Paul Hassan, Helle Clausen, Henrik
Abstract	The glycosylation of serine and threonine residues during mucin-type O-linked protein glycosylation is carried out by a family of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferases (GalNAc-transferase). Previously two members, GalNAc-T1 and −T2, have been isolated and the genes cloned and characterized. Here we report the cDNA cloning and expression of a novel GalNAc-transferase termed GalNAc-T3. The gene was isolated and cloned based on the identification of a GalNAc-transferase motif (61 amino acids) that is shared between GalNAc-T1 and −T2 as well as a homologous Caenorhabditis elegans gene. The cDNA sequence has a 633-amino acid coding region indicating a protein of 72.5 kDa with a type II domain structure. The overall amino acid sequence similarity with GalNAc-T1 and −T2 is approximately 45%; 12 cysteine residues that are shared between GalNAc-T1 and −T2 are also found in GalNAc-T3. GalNAc-T3 was expressed as a soluble protein without the hydrophobic transmembrane domain in insect cells using a Baculo-virus vector, and the expressed GalNAc-transferase activity showed substrate specificity different from that previously reported for GalNAc-T1 and −T2. Northern analysis of human organs revealed a very restricted expression pattern of GalNAc-T3.
Related Links	http://www.jbc.org/content/271/29/17006.abstract
Ending Page	17012
Starting Page	17006
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	29
Volume Number	271
DOI	10.1074/jbc.271.29.17006
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1996-07-19
Access Restriction	Open
Subject Keyword	Carbohydrates, Lipids, and Other Natural Products
Alternative Title	cDNA Cloning and Expression of a Novel Human UDP-N-acetyl-α-D-galactosamine
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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