NDLI: The Leucyl/Phenylalanyl-tRNA-Protein Transferase OVEREXPRESSION AND CHARACTERIZATION OF SUBSTRATE RECOGNITION, DOMAIN STRUCTURE, AND SECONDARY STRUCTURE

Content Provider	Journal of Biological Chemistry (JBC)
Author	Abramochkin, Georgi Shrader, Thomas E.
Abstract	Previous work has shown that, in the bacterium Escherichia coli, the aat gene is essential for the degradation of proteins bearing amino-terminal Arg and Lys residues via the N-end rule pathway of protein degradation. We now show that the aat gene encodes directly the leucyl/phenylalanyl-tRNA-protein transferase (L/F-transferase). This enzyme catalyzes the transfer of Leu, Phe, and, less efficiently, Met and Trp, from aminoacyl-tRNAs, to the amino terminus of acceptor proteins. We have used the cloned aat gene to overexpress and purify an affinity tagged L/F-transferase. The recombinant L/F-transferase is as active as the previously purified wild type enzyme and contains no detectable RNA component. We have used the recombinant enzyme to demonstrate that both the solubility and substrate specificity, for aminoacyl-tRNA substrates, of the L/F-transferase are dependent on ionic strength conditions and that the modified nucleotides found in natural tRNAs are not essential for recognition by the enzyme. Limited digestion of the L/F-transferase with trypsin removes the proline rich NH2 terminus of the enzyme identifying a globular core, and circular dichroism demonstrates that the L/F-transferase is predominantly α-helical. Finally, a region of sequence conservation between the L/F-transferase and the NH2-terminal protein acetylases has been identified.
Related Links	http://www.jbc.org/content/270/35/20621.abstract
Ending Page	20628
Starting Page	20621
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	35
Volume Number	270
DOI	10.1074/jbc.270.35.20621
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1995-09-01
Access Restriction	Open
Subject Keyword	Enzymology
Alternative Title	The Leucyl/Phenylalanyl-tRNA-Protein Transferase
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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