NDLI: Hyaluronic Acid Synthesis Operon (has) Expression in Group A Streptococci

Content Provider	Journal of Biological Chemistry (JBC)
Author	Crater, Dinene L. Rijn, I. Van De
Abstract	The has operon is composed of three genes, hasA, hasB, and hasC that encode hyaluronate synthase, UDP-glucose dehydrogenase, and presumptively UDP-glucose pyrophosphorylase, respectively. Expression of the has operon was shown to be required for the synthesis of the hyaluronic acid capsule in group A streptococci. Previous studies indicated that some group A and group C streptococcal strains produce the hyaluronic acid capsule, while others do not. In addition, it was observed that encapsulated strains cultured in stationary phase of growth lose the hyaluronic acid capsule. Therefore, the molecular mechanisms controlling the expression of the hyaluronic acid capsule in group A streptococci was investigated. In this study, it was determined that all encapsulated and unencapsulated strains of group A streptococci as well as encapsulated group C streptococci analyzed possess the has operon locus. The acapsular phenotype was accounted for by the absence of hyaluronate synthase activity in the membrane and not the production of extracellular hyaluronidase. A has operon mRNA transcript was not expressed by unencapsulated strains of group A streptococci, whereas encapsulated strains of group A streptococci grown to mid to late exponential phase produced the hyaluronate capsule, as well as has operon mRNA. However, as the streptococci entered the stationary phase of growth, they became acapsular and this was concomitant with the loss of has operon mRNA transcript. These results were confirmed by primer extension analyses of RNA isolated from encapsulated and unencapsulated strains of group A streptococci as well as RNA prepared from encapsulated strains cultured in exponential and stationary phases of growth. Thus, the loss of has operon mRNA in unencapsulated group A streptococci, as well as growth phase regulation occurs at the previously mapped has operon promoter. These data suggested that the synthesis of the hyaluronic acid capsule for group A streptococci may be controlled by transcriptional mechanisms.
Related Links	http://www.jbc.org/content/270/31/18452.abstract
Ending Page	18458
Starting Page	18452
Page Count	7
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	31
Volume Number	270
DOI	10.1074/jbc.270.31.18452
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1995-08-04
Access Restriction	Open
Subject Keyword	Carbohydrates, Lipids, and Other Natural Products
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Expression Cloning and Molecular Characterization of HAS Protein, a Eukaryotic Hyaluronan Synthase

Molecular characterization of hasA from an operon required for hyaluronic acid synthesis in group A streptococci.

A Human Polysialyltransferase Directs in Vitro Synthesis of Polysialic Acid

A Human STX cDNA Confers Polysialic Acid Expression in Mammalian Cells

Molecular Directionality of Polysaccharide Polymerization by thePasteurella multocida Hyaluronan Synthase

Evolution of the Hyaluronic Acid Synthesis (has) Operon in Streptococcus zooepidemicus and Other Pathogenic Streptococci

Khafrefungin, a Novel Inhibitor of Sphingolipid Synthesis

Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity.

Molecular Cloning and Expression of Rat Squalene Epoxidase

Hyaluronic Acid Synthesis Operon (has) Expression in Group A Streptococci

Similar Documents

Expression Cloning and Molecular Characterization of HAS Protein, a Eukaryotic Hyaluronan Synthase

Molecular characterization of hasA from an operon required for hyaluronic acid synthesis in group A streptococci.

A Human Polysialyltransferase Directs in Vitro Synthesis of Polysialic Acid

A Human STX cDNA Confers Polysialic Acid Expression in Mammalian Cells

Molecular Directionality of Polysaccharide Polymerization by thePasteurella multocida Hyaluronan Synthase

Evolution of the Hyaluronic Acid Synthesis (has) Operon in Streptococcus zooepidemicus and Other Pathogenic Streptococci

Khafrefungin, a Novel Inhibitor of Sphingolipid Synthesis

Molecular characterization of hasB from an operon required for hyaluronic acid synthesis in group A streptococci. Demonstration of UDP-glucose dehydrogenase activity.

Molecular Cloning and Expression of Rat Squalene Epoxidase

Hyaluronic Acid Synthesis Operon (has) Expression in Group A Streptococci