NDLI: Transcription of adenovirus-2 major late promoter inhibited by monoclonal antibody directed against RNA polymerases IIO and IIA.

Content Provider	Journal of Biological Chemistry (JBC)
Author	Dahmus, M. E. Kedinger, C.
Abstract	Monoclonal antibody directed against RNA polymerases IIO and IIA completely inhibits specific transcription from the major late promoter of adenovirus-2. At comparable antibody concentrations, only a slight inhibition of polymerase III transcription of VA RNA I is observed. Inhibition of polymerase II transcription is specific for monoclonal IgM directed against RNA polymerase II. High concentrations of control IgM do not inhibit transcription. Specific transcription from the conalbumin and ovalbumin promoters is also inhibited by polymerase-specific monoclonal IgM but not control IgM. These results suggest that the in vitro transcription of the conalbumin, ovalbumin, and adenovirus-2 major late promoter is catalyzed by RNA polymerase(s) IIO and/or IIA but not RNA polymerase IIB. However, transcription from calf thymus DNA, known to contain nicks, is not inhibited by polymerase specific monoclonal antibody. Thus, the monoclonal antibody does not interfere with initiation or elongation of RNA polymerase from nonpromoter sites but does appear to selectively inhibit transcription from promoter sites.
Related Links	http://www.jbc.org/content/258/4/2303.abstract
Ending Page	2307
Starting Page	2303
Page Count	5
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	4
Volume Number	258
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1983-02-25
Access Restriction	Open
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The major late promoter of adenovirus-2 is accurately transcribed by RNA polymerases IIO, IIA, and IIB.

Monoclonal antibody specific for calf thymus RNA polymerases IIO and IIA.

RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter.

In vitro transcription initiation by purified RNA polymerase II within the adenovirus 2 major late promoter region.

The interaction of RNA polymerase II with the adenovirus-2 major late promoter is precluded by phosphorylation of the C-terminal domain of subunit IIa.

Abortive initiation by RNA polymerase II in vitro at the adenovirus 2 major late promoter.

In vitro transcription from the adenovirus 2 major late promoter utilizing templates truncated at promoter-proximal sites.

Chromatin Is Permissive to TATA-binding Protein (TBP)-mediated Transcription Initiation

Interaction of CAP sequence site binding factor and transcription factor IID preceding and following binding to the adenovirus 2 major late promoter.

Transcription of adenovirus-2 major late promoter inhibited by monoclonal antibody directed against RNA polymerases IIO and IIA.

Similar Documents

The major late promoter of adenovirus-2 is accurately transcribed by RNA polymerases IIO, IIA, and IIB.

Monoclonal antibody specific for calf thymus RNA polymerases IIO and IIA.

RNA polymerases IIA and IIO have distinct roles during transcription from the TATA-less murine dihydrofolate reductase promoter.

In vitro transcription initiation by purified RNA polymerase II within the adenovirus 2 major late promoter region.

The interaction of RNA polymerase II with the adenovirus-2 major late promoter is precluded by phosphorylation of the C-terminal domain of subunit IIa.

Abortive initiation by RNA polymerase II in vitro at the adenovirus 2 major late promoter.

In vitro transcription from the adenovirus 2 major late promoter utilizing templates truncated at promoter-proximal sites.

Chromatin Is Permissive to TATA-binding Protein (TBP)-mediated Transcription Initiation

Interaction of CAP sequence site binding factor and transcription factor IID preceding and following binding to the adenovirus 2 major late promoter.

Transcription of adenovirus-2 major late promoter inhibited by monoclonal antibody directed against RNA polymerases IIO and IIA.