NDLI: Inhibition of Membrane-bound Adenosine Triphosphatase and of Cation Transport in Streptococcus faecalis by N,N'-Dicyclohexylcarbodiimide

Content Provider	Journal of Biological Chemistry (JBC)
Author	Harold, Franklin M. Baarda, James R. Baron, Carl Abrams, Adolph
Abstract	N,N'-Dicyclohexylcarbodiimide (DCCD) was found to be a potent inhibitor of the membrane-bound ATPase of Streptococcus faecalis but did not inhibit the solubilized form of the enzyme. Inhibited membrane-bound ATPase was reactivated by releasing the enzyme from the membrane. Conversely, sensitivity to DCCD was restored by reconstituting the ATPase-membrane complex from solubilized ATPase and depleted membranes. The results indicate that DCCD reacts covalently with a membrane component and inhibits the enzyme indirectly, perhaps by means of a transmitted effect on its conformation.When added to intact cells, DCCD reduced the rate of glycolysis yet the ATP content of the cells was as high in the presence of the inhibitor as in its absence. DCCD strongly inhibited the degradation of ATP which ensued when glycolysis was stopped, either by removal of glucose or by addition of iodoacetate. The results suggest that DCCD inhibits ATPase in vivo, just as it does in isolated membrane preparations. The inhibition of over-all glycolysis may be secondary to the inhibition of ATP degradation.DCCD also inhibited a number of energy-dependent transport processes, including the accumulation of K+ by exchange for H+ and Na+ and the uptake of phosphate and alanine. Inhibition was observed whether glucose or arginine served as energy source. The findings are consistent with the hypothesis that the membrane-bound ATPase of S. faecalis mediates energy transductions involved in membrane transport; inhibition of transport by DCCD would be a consequence of inhibition of the ATPase. Alternatively, DCCD may bind to sites on the membrane to inhibit indirectly both transport and ATP hydrolysis.
Related Links	http://www.jbc.org/content/244/9/2261.abstract
Ending Page	2268
Starting Page	2261
Page Count	8
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	9
Volume Number	244
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1969-05-10
Access Restriction	Open
Subject Keyword	OXIDATION-REDUCTION AND BIOENERGETICS
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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