NDLI: The Chemistry and Molecular Architecture of the Cell Walls of Penicillium chrysogenum

Content Provider	Journal of Biological Chemistry (JBC)
Author	Troy, Frederic A. Koffler, Henry
Abstract	Treatment of 14C-uniformly labeled cell walls of Penicillium chrysogenum with chitobiase-containing chitinase resulted in 73% solubilization. The solubilized components were isolated, identified, and determined quantitatively. N-Acetylglucosamine (GlcNAc) and N,N'-diacetylchitobiose ((GlcNAc)2) accounted for 42% of the cell wall. Since kinetic analysis showed that GlcNAc arose from (GlcNAc)2, at least 42% of the cell wall contains GlcNAc in β-d-(1 → 4)-glycosidic linkage. When the same experiment was performed with a chitobiase-free chitinase preparation nearly identical results were obtained, except, as expected, essentially all of the GlcNAc was present as (GlcNAc)2. Small amounts of glucose and laminaritriose were also released. In addition, a nondialyzable component, Fraction 5A, accounting for 14% of the wall, was isolated. This fraction, devoid of GlcNAc, contains 20 times the amount of galactose and 2 times the amount of mannose with respect to glucose found in cell wall hydrolysates. An additional 13% of the wall was solubilized when the cell wall residue, following treatment with chitinase, was treated with chitinase-free β-d-(1 → 3)-glucanase. Therefore, 86% of the wall was solubilized when treated first with chitinase, then with β-d-(1 → 3)-glucanase.Initial treatment of cell walls with β-d-(1 → 3)-glucanase resulted in 48% solubilization. From an analysis of the solubilized components the conclusion was reached that at least 40% of the wall contains glucose in β-d-(1 → 3)-glycosidic linkage. An additional 44% of the wall was solubilized when the residue, following treatment with β-d-(1 → 3)-glucanase, was treated with chitinase. Thus, 92% of the wall was solubilized when treated first with β-d-(1 → 3)-glucanase, then with chitinase.The observations that β-d-(1 → 3)-glucanase-free chitinase released in addition to GlcNAc and (GlcNAc)2 also glucose, laminaritriose, and a fraction containing mannose, galactose, and glucose (Fraction 5A) and that treatment of this fraction with β-d-(1 → 3)-glucanase released glucose and laminaribiose suggest that glucose may exist in cell walls in one or more forms other than the major glucan. From the available data it is not clear whether Fraction 5A contains one or several polymers.An analysis of the monomeric cell wall constituents released by acid hydrolysis is in excellent agreement with the results obtained by enzymatic digestion. These results showed the cell wall to contain about 48% glucosamine and about 52% neutral hexoses, which consisted of glucose, galactose, and mannose in the molar proportion of 10:1:2. Thus, if 40% of the wall is glucose, then galactose and mannose account for 4% and 8% of the wall, respectively.
Related Links	http://www.jbc.org/content/244/20/5563.abstract
Ending Page	5576
Starting Page	5563
Page Count	14
File Format	HTM / HTML PDF
ISSN	00219258
Journal	Journal of Biological Chemistry (JBC)
Issue Number	20
Volume Number	244
e-ISSN	1083351X
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	1969-10-25
Access Restriction	Open
Subject Keyword	CHEMISTRY AND METABOLISM OF MACROMOLECULES
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls XIX. ISOPRENOID ALCOHOL PHOSPHOKINASE

Organic acid metabolism of Penicillium chrysogenum. 1. Lactate and acetate

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls XI. FORMATION OF THE ISOGLUTAMINE AMIDE GROUP IN THE CELL WALLS OF STAPHYLOCOCCUS AUREUS

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls II. PHOSPHOLIPID CARRIERS IN THE REACTION SEQUENCE

Isolation and Study of the Chemical Structure of Low Molecular Weight Glycopeptides from Micrococcus lysodeikticus Cell Walls

Isolation and Study of the Chemical Structure of a Disaccharide from Micrococcus lysodeikticus Cell Walls

A STUDY OF LIPIDES OF PENICILLIUM CHRYSOGENUM

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls IV. INCORPORATION OF GLYCINE IN MICROCOCCUS LYSODEIKTICUS

Biochemistry of filamentous fungi i. Oxdative metabolism of glucose by penicillium chrysogenum' (1951).

The Chemistry and Molecular Architecture of the Cell Walls of Penicillium chrysogenum

Similar Documents

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls XIX. ISOPRENOID ALCOHOL PHOSPHOKINASE

Organic acid metabolism of Penicillium chrysogenum. 1. Lactate and acetate

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls XI. FORMATION OF THE ISOGLUTAMINE AMIDE GROUP IN THE CELL WALLS OF STAPHYLOCOCCUS AUREUS

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls II. PHOSPHOLIPID CARRIERS IN THE REACTION SEQUENCE

Isolation and Study of the Chemical Structure of Low Molecular Weight Glycopeptides from Micrococcus lysodeikticus Cell Walls

Isolation and Study of the Chemical Structure of a Disaccharide from Micrococcus lysodeikticus Cell Walls

A STUDY OF LIPIDES OF PENICILLIUM CHRYSOGENUM

Biosynthesis of the Peptidoglycan of Bacterial Cell Walls IV. INCORPORATION OF GLYCINE IN MICROCOCCUS LYSODEIKTICUS

Biochemistry of filamentous fungi i. Oxdative metabolism of glucose by penicillium chrysogenum' (1951).

The Chemistry and Molecular Architecture of the Cell Walls of Penicillium chrysogenum