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| Content Provider | Journal of Biological Chemistry (JBC) |
|---|---|
| Author | Nixon, J. E. Putz, G. R. Porter, John W. |
| Abstract | The synthesis of triacetic acid lactone (TAL) is effected by purified soluble pigeon liver fatty acid synthetase. This enzyme system synthesizes TAL from acetyl coenzyme A and malonyl coenzyme A in the absence of TPNH, and synthesizes palmitic acid in the presence of TPNH. The major product of the reaction in the absence of TPNH is TAL, and not free triacetic acid. Presumably triacetic acid, bound as a thioester to the 4'-phosphopantetheine prosthetic group of the fatty acid synthetase, is an intermediate in the formation of TAL. The latter compound is formed from 1 mole of acetyl-CoA and 2 moles of malonyl-CoA in the sequence, acetate-malonate-malonate (proceeding left to right from the methyl end of the molecule). The synthesis of TAL is inhibited by either TPN+ or TPNH. The very strong inhibition by both forms of the nucleotide implies that TAL is not synthesized in vivo by the avian soluble fatty acid synthetase. |
| Related Links | http://www.jbc.org/content/243/20/5471.abstract |
| Ending Page | 5478 |
| Starting Page | 5471 |
| Page Count | 8 |
| File Format | HTM / HTML PDF |
| ISSN | 00219258 |
| Journal | Journal of Biological Chemistry (JBC) |
| Issue Number | 20 |
| Volume Number | 243 |
| e-ISSN | 1083351X |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 1968-10-25 |
| Access Restriction | Open |
| Subject Keyword | ENZYMOLOGY |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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