NDLI: Combined assay of Circulating Tumor DNA and Protein Biomarkers for early noninvasive detection and prognosis of Non-Small Cell Lung Cancer

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Combined assay of Circulating Tumor DNA and Protein Biomarkers for early noninvasive detection and prognosis of Non-Small Cell Lung Cancer

Combined assay of Circulating Tumor DNA and Protein Biomarkers for early noninvasive detection and prognosis of Non-Small Cell Lung Cancer

Content Provider	Ivyspring International Publisher
Author	Yin, Jian-xin Hu, Wen-wei Gu, Hua Fang, Jian-min
Editor	Arena, Francis P.
Abstract	Purpose: Early diagnosis of lung cancer is critical to curtailing cancer-related deaths. We aimed to develop a highly sensitive assay for the analysis of circulating tumor DNA (ctDNA) to detect non-small cell lung cancer (NSCLC) in the early stages.Materials and Methods: We detected EGFR and KRAS mutations in paired plasma and tumor tissue samples from 147 NSCLC patients. Of these, EGFR/KRAS ctDNA mutations and protein biomarkers were comparatively analyzed in 87 individuals. In addition, tissue samples of 20 patients were subjected to repeat multi-gene detection, and pre- and post-operative paired samples of 28 patients were subjected to multi-gene detection. Clinical information was obtained to complement the prognostic value of the combined assay results and post-operative new ctDNA mutation status.Results: EGFR/KRAS mutations were highly consistent in ctDNA and tumor DNA. Combining the detection of EGFR and KRAS mutations in ctDNA with the detection of protein biomarkers increased cancer detection sensitivity to 74.7% (65/87). None of the healthy controls tested positive using the combined assay (100% specificity). Combined assay results independently associated with recurrence-free survival. Post-operative new ctDNA mutation status independently associated with overall survival and recurrence-free survival.Conclusion: The detection of ctDNA may be exploited for early diagnosis of NSCLC, as highlighted by the developed assay. Further, the combined assay results and post-operative new ctDNA mutation status are promising prognostic indicators in NSCLC patients.
Related Links	https://www.jcancer.org/v12p1258.pdf
Ending Page	1269
Starting Page	1258
File Format	PDF
ISSN	18379664
DOI	10.7150/jca.49647
Journal	Journal of Cancer
PubMed reference number	33442424
Issue Number	4
PubMed Central reference number	PMC7797658
Volume Number	12
Language	English
Publisher	Ivyspring International Publisher
Publisher Date	2021-01-01
Access Restriction	Open
Subject Keyword	Non-Small Cell Lung Cancer Next-generation sequencing Cancer early detection Circulating tumor DNA Tumor biomarkers Research Paper
Content Type	Text
Resource Type	Article
Subject	Medicine
Journal Impact Factor	3.2

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