Terpyridine Oxovanadium(IV) Complexes Of Phenanthroline Bases For Cellular Imaging And Photocytotoxicity In HeLa Cells

Content Provider	Indian Institute of Science (IISc)
Author	Banik, Bhabatosh Sasmal, Pijus K. Roy, Sovan Majumdar, Ritankar Dighe, Rajan R. Chakravarty, Akhil R.
Copyright Year	2011
Abstract	Oxovanadium(IV) complexes VO(N-N-N)(N-N)](NO3)(2) (1-4) of (4'-phenyl)-2,2': 6',2 `'-terpyridine (ph-tpy in 1 and 2) or (4'-pyrenyl)-2,2':6',2 `'-terpyridine (py-tpy in 3 and 4) having N-N as 1,10-phenanthroline (phen in 1 and 3) or dipyrido3,2-a:2',3'-c]phenazine (dppz in 2 and 4) are prepared and characterized. The crystal structure of 1 has VO2+ group in VN5O coordination geometry. The terpyridine ligand coordinates in a meridional binding mode. The phen ligand displays a chelating mode of binding with an N-donor site trans to the vanadyl oxo group. The complexes show a d-d band in the range of 710-770 nm in aqueous DMF (4:1 v v). The complexes exhibit an irreversible V-IV V-III redox response near -1.0 V vs. SCE in aqueous DMF 0.1 M KCl. The complexes bind to CT DNA giving K-b values within 3.5 x 10(5) to 1.2 x 10(6) M-1. The complexes show poor chemical nuclease activity in dark. Complexes 2-4 show photonuclease activity in UV-A light of 365 nm forming O-1(2) and (OH)-O-center dot. Complex 4 shows DNA photocleavage activity at near-IR light of 785 nm forming (OH)-O-center dot radicals. Complexes 2 and 4 show significant photocytotoxicity in HeLa cancer cells. Uptake of the complexes in HeLa cells, studied by fluorescence imaging, show predominantly cytosolic localization inside the cells.
File Format	PDF
Journal	PeerReviewed
Language	English
Publisher	John Wiley and Sons
Publisher Date	2011-03-01
Access Restriction	Authorized
Subject Keyword	Molecular Reproduction, Development & Genetics (formed by the merger of DBGL and CRBME) Inorganic & Physical Chemistry
Content Type	Text
Resource Type	Article