NDLI: Transfection Of Mycobacterium Smegmatis SN2 With Mycobacteriophage I3 DNA

Content Provider	Indian Institute of Science (IISc)
Author	Karnik, Sadashiva S. Gopinathan, K. P.
Copyright Year	1983
Abstract	Mycobacterium smegmatis SN2 does not exhibit natural competence for the uptake of phage I3 DNA. Competence can artificially be induced by treatment with glycine or $CaCl_2$, and the combination of both is even more effective. The efficiency of transfection can be improved by inclusion of protamine sulphate and heterologous RNA in the system. From $^{32}P$ DNA uptake studies the major barrier for the entry of DNA has been found to be the complex cell wall. The efficiency of transfection calculated on the basis of fraction of DNA which has entered the cell is comparable to that of other bacterial systems. The phage development takes a longer time (7 h for one cycle) after transfection, as compared to infection (4 h)
File Format	PDF
Journal	PeerReviewed
Language	English
Publisher	Springer Berlin / Heidelberg
Publisher Date	1983-12-01
Access Restriction	Authorized
Subject Keyword	Microbiology & Cell Biology
Content Type	Text
Resource Type	Article

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