Copper(I) Complexes Of Modified Nucleobases And Vitamin B3 As Potential Chemotherapeutic Agents: In Vitro And In Vivo Studies

Content Provider	Indian Institute of Science (IISc)
Author	Sanghamitra, N. J. M. Adwankar, M. K. Juvekar, A. S. Khurajjam, V. Wycliff, C. Samuelson, A. G.
Copyright Year	2011
Abstract	Three new complexes of Cu(I) have been synthesized using ancillary ligands like thiopyrimidine (tp) a modified nucleobase, and nicotinamide (nie) or vitamin B3, and characterized by spectroscopy and X-ray crystallography. In vitro cytotoxicity studies of the complexes on various human cancer cell lines such as Colo295, H226, HOP62, K562, MCF7 and T24 show that Cu(PPh3)(2)(tp)Cl] and Cu(PPh3)(2)(tp)ClO4 (2) have in vitro cytotoxicity comparable to cisplatin. Complex Cu(nic)(3)PPh3]ClO4 (3) is non-toxic and increases the life span by about 55 % in spontaneous breast tumor model. DNA binding and cleavage studies show that complex (3) binds to calf thymus DNA with an apparent binding constant of 5.9 x 10(5)M and completely cleaves super-coiled DNA at a concentration of 400 mu M, whereas complexes (1) and (2) do not bind DNA and do not show any cleavage even at 1200 mu M. Thus, complex (3) may exhibit cytotoxicity Via DNA cleavage whereas the mechanism of cytotoxicity of (1) and (2) probably involves a different pathway.
File Format	PDF
Journal	PeerReviewed
Language	English
Publisher	National Institute of Science Communication and Information Resources
Publisher Date	2011-04-01
Access Restriction	Authorized
Subject Keyword	Inorganic & Physical Chemistry
Content Type	Text
Resource Type	Article