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| Content Provider | IET Digital Library |
|---|---|
| Author | Ma, Wenying Tang, Shi Wei, Yaohua Xie, Guangzhong |
| Abstract | A simple and stable method to detect dimethyl methylphosphonate (DMMP) was studied. Through direct monitoring of acetylcholinesterase (AChE) inhibition without histological substrate, the concentration of DMMP was sensitively detected. In the experimental setup, a liquid–gas interface was developed to improve the oscillation stability of quartz crystal microbalances (QCMs) and to prevent protein denaturation of AChE. AChE was immobilised on the surface of gold electrodes on QCM by creating a cross-linked gel with glutaraldehyde. Experimental results showed that the prepared biosensor had a frequency fall of ∼4 Hz when a concentration of 1 ng/ml increment occurred for liquid DMMP. Furthermore, the detection was repeatable and selective to only DMMP. Frequency response to DMMP in gas phase was also studied. The considerable frequency response and its complete recovery showed that the sensor had wide application potential in attack defence and public safety construction. |
| Starting Page | 113 |
| Ending Page | 116 |
| Page Count | 4 |
| Volume Number | 12 |
| e-ISSN | 17500443 |
| Issue Number | Issue 2, Feb (2017) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/mnl/12/2 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/mnl.2016.0497 |
| Journal | Micro & Nano Letters |
| Publisher Date | 2017-02-01 |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | Acetylcholine Esterase Sensitive Film AChE Immobilisation Attack Defence BioMEMS Biomolecular Interactions Biosensing Biosensor Charge Transfer Complexes Chemical Analysis And Related Physical Method of Analysis Chemical Sensor Chemical Variables Measurement Cross-linked Gel Dimethyl Methylphosphonate DMMP Detection Electrode Enzymes Frequency Response Gas Sonsor Glutaraldehyde Gold Gold Electrodes Liquid-gas Interface MEMS And NEMS Device Technology Micromechanical And Nanomechanical Device And System Microsensor Microsensors And Nanosensors Molecular Biophysics National Security Oscillation Stability Protein Denaturation Public Safety Construction QCM Transducer Quartz Crystal Microbalances Transducers |
| Content Type | Text |
| Resource Type | Article |
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