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| Content Provider | IET Digital Library |
|---|---|
| Author | You, Jiaqi Ma, Long He, Yu Ge, Yili Song, Gongwu Zhou, Jiangang |
| Abstract | This work has assembled ZnSe:Mn/ZnS quantum dots (QDs)-monoclonal anti-microcystin-Leucine Arginine (anti-MC-LR) antibody bioconjugates as a room temperature phosphorescence (RTP) probe using 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide/N-Hydroxysuccinimide chemistry for detecting MC-LR. Once the specific interactions occurred between these MCs and their antibodies on the surface of ZnSe:Mn/ZnS QDs, the RTP of ZnSe:Mn/ZnS QDs was quenched with the increased concentration of MC-LR. Under the optimised conditions, the linear range of MC-LR was determined to be 0.9–8.8 nM. The linear relationship between (P 0–P)/P 0 and concentration of MC-LR were observed with the regression equation (P 0–P)/P 0 = 0.03908C + 0.0456 with the detection limit of 0.038 nM. The developed RTP immunoassay favoured environmental applications since the interference from autofluorescence and scattering light was greatly eliminated. |
| Starting Page | 892 |
| Ending Page | 896 |
| Page Count | 5 |
| Volume Number | 14 |
| e-ISSN | 17500443 |
| Issue Number | Issue 8, Jul (2019) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/mnl/14/8 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/mnl.2018.5690 |
| Journal | Micro & Nano Letters |
| Publisher Date | 2019-07-24 |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | 1-ethyl-3-(3-dimethyllaminopropyl) Carbodiimide-N-Hydroxysuccinimide Chemistry AntiMC-LR Antibody Detection Assembling Autofluorescence Biological Technique Biophysical Instrumentation And Technique Chemical Sensor Chemical Variables Measurement II-VI SemiConductor Interference Light Scattering Manganese Microassembly Technique Microcystin Detection Monoclonal Antimicrocystin-Leucine Arginine Antibody Phosphorescence Probability Theory QD Quantum Dot Quenching Regression Analysis Regression Equation Room Temperature Phosphorescence Immunoassay Probe RTP Immunoassay Probe Semiconductor Quantum Dots Statistics Stochastic Linearised SCUC Temperature 293 K to 298 K Wide Band Gap SemiConductor Zinc Compound ZnSe:Mn-ZnS |
| Content Type | Text |
| Resource Type | Article |
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