NDLI: Positive selection on D-lactate dehydrogenases of Lactobacillus delbrueckii subspecies bulgaricus

Content Provider	IET Digital Library
Author	Zhang, Jifeng Gong, Guangyu Wang, Xiao Zhang, Hao Tian, Weidong
Abstract	Lactobacillus delbrueckii has been widely used for yogurt fermentation. It has genes encoding both D- and L-type lactate dehydrogenases (LDHs) that catalyse the production of L(+) or D(−) stereoisomer of lactic acid. D-lactic acid is the primary lactate product by L. delbrueckii, yet it cannot be metabolised by human intestine. Since it has been domesticated for long time, an interesting question arises regarding to whether the selection pressure has affected the evolution of both L-LDH and D-LDH genes in the genome. To answer this question, in this study the authors first investigated the evolution of these two genes by constructing phylogenetic trees. They found that D-LDH-based phylogenetic tree could better represent the phylogenetic relationship in the acidophilus complex than L-LDH-based tree. They next investigated the evolutions of LDH genes of L. delbrueckii at amino acid level, and found that D-LDH gene in L. delbrueckii is positively selected, possibly a consequence of long-term domestication. They further identified four amino acids that are under positive selection. One of them, V261, is located at the centre of three catalytic active sites, indicating likely functional effects on the enzyme activity. The selection from the domestication process thus provides direction for future engineering of D-LDH.
Starting Page	172
Ending Page	179
Page Count	8
ISSN	17518849
Volume Number	9
e-ISSN	17518857
Issue Number	Issue 4, Aug (2015)
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/iet-syb/9/4
Alternate Webpage(s)	https://digital-library.theiet.org/content/journals/10.1049/iet-syb.2014.0056
Journal	IET Systems Biology
Publisher Date	2015-08-01
Access Restriction	Open
Rights Holder	© The Institution of Engineering and Technology
Subject Keyword	Acidophilus Complex Amino Acids Biochemistry Biological Technique Biomolecular Structure, Configuration, Conformation, And Active Sites Biophysical Instrumentation And Technique Biotechnology Industry D-lactate Dehydrogenases D-LDH Genes Enzyme Activity Enzymes Evolution (biological) Fermentation Food Industry Food Product Genetics Genomic Technique Isomerism L-LDH Genes Lactic Acid Stereoisomer Lactobacillus Delbrueckii Macromolecular Configuration (bonds, Dimensions) Microorganisms Molecular Biophysics Molecular Configurations Phylogenetic Trees Physical Chemistry of Biomolecular Solutions And Condensed State Product And Commodities Streptococcus Thermophilus Three-dimensional Structure Yogurt Fermentation
Content Type	Text
Resource Type	Article
Subject	Cell Biology Genetics Molecular Biology Modeling and Simulation Biotechnology

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