NDLI: Merocyanine 540-mediated photodynamic therapy against Staphylococcus aureus planktonic and biofilm cells

Content Provider	IEEE Xplore Digital Library
Author	Hsiao-Yin Lin Chin-Tin Chen Ching-Tsan Huang
Copyright Year	2004
Description	Author affiliation: Inst. of Microbiol. & Biochem., Nat. Taiwan Univ., Taipei, Taiwan (Hsiao-Yin Lin)
Abstract	Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a photosensitizer, merocyanine 540 (MC 540) was investigated. For the planktonic experiments, the antimicrobial activity was enhanced with the increasing light dose, but an increase in light dose could not further improve the antimicrobial activity if the maximum excitation level attainable lay below the necessary minimum threshold level. A complete inactivation was achieved when the excitation level of MC 540 lay somewhere above the threshold level. The relationship between antimicrobial activity and the excitation level of MC 540 revealed that the more MC 540 was excited, the more S. aureus cells were killed. For the biofilm experiments, the antimicrobial activity was enhanced with the increasing light dose. No viable cells were detected when exposed to 15 /spl mu/g/ml MC 540 and a light dose of 600 J/cm/sup 2/ or 20 /spl mu/g/ml MC 540 and a light dose of 450 J/cm/sup 2/. The results from both planktonic and biofilm experiments suggest that the antimicrobial activity of photodynamic inactivation against S. aureus is closely related to the excitation level of MC 540.
Starting Page	84
Ending Page	85
File Size	141756
Page Count	2
File Format	PDF
ISBN	0780386760
DOI	10.1109/APBP.2004.1412290
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-12-14
Publisher Place	Taiwan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microorganisms Medical treatment Immune system Biological materials
Content Type	Text
Resource Type	Article

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