NDLI: Electrical cell-substrate impedance sensing (ECIS) based biosensor for characterization of DF-1 cells

Content Provider	IEEE Xplore Digital Library
Author	Siddiquei, H.R. Nordin, A.N. Ibrahimy, M.I. Arifin, M.A. Sulong, N.H. Mel, M. Voiculescu, I.
Copyright Year	2010
Description	Author affiliation: Biotechnology Engineering Department, Kulliyah of Engineering, International Islamic University, Kuala Lumpur, Malaysia (Arifin, M.A.; Sulong, N.H.; Mel, M.) \|\| Dept. of Mechanical Engineering, City College of New York, 160 Convent Avenue, New York, USA (Voiculescu, I.) \|\| ECE Department, Kulliyah of Engineering, International Islamic University, Kuala Lumpur, Malaysia (Siddiquei, H.R.; Nordin, A.N.; Ibrahimy, M.I.)
Abstract	Electric cell-substrate impedance sensing (ECIS) method can be used as a valuable tool for real time monitoring of cell behavior such as attachment, mobility, and growth. Changes in impedance of the cells due to growth and attachment can be modeled as an equivalent circuit consisting of resistors and capacitors of both the cell culture media and the cells. In this work, a biosensor which measures the impedance in DF-1 cells (derived from chicken embroyonic fibroblasts and CEF cells) are presented. The biosensor consists of a Teflon cell holder and two gold electrodes. Experimental measurements were conducted using DF-1 cells cultured in DMEM media. Two different experiments were conducted namely; the control experiment (holder contains only DMEM media) and the cell experiment (holder contains both DMEM media and cells). The biosensor was placed in an incubator with optimum settings for cell growth. Impedance measurements were sampled at six hour intervals. Based on these measurements the resistance and capacitance change due to the growth of the DF-1 cells were calculated. It was observed that significant change in resistance and capacitance values occurs in the first six hours, where cell growth and attachment is most active. After this period of time, the cells become confluent and capacitance values become saturated.
Starting Page	1
Ending Page	4
File Size	552408
Page Count	4
File Format	PDF
ISBN	9781424462339
e-ISBN	9781424462353
DOI	10.1109/ICCCE.2010.5556772
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-05-11
Publisher Place	Malaysia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Electrical cell impedance sensing (ECIS) Impedance measurement impedance spectroscopy electrode impedance animal cell Media Capacitance DF-1 Impedance Biosensors
Content Type	Text
Resource Type	Article

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