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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Gryczynski, Zygmunt Matveeva, Evgenia Shtoyko, Tanya Goldys, Ewa Schetz, John Borejdo, Julian Calander, Nils Gryczynski, Ignacy |
| Copyright Year | 2006 |
| Description | Author affiliation: University of Texas at Tylor, Tyler TX. (Shtoyko, Tanya) || Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107; Department of Cell Biology and Genetics, University of North Texas Health Science Center, Fort Worth, TX 76107. (Gryczynski, Ignacy) || Department of Molecular Biology and Immunology, University of North Texas Health Science Center, Fort Worth, TX 76107. (Gryczynski, Zygmunt; Matveeva, Evgenia; Borejdo, Julian) || Division of Information and Communication Sciences, Macquarie University, Sydney 2109 NSW Australia; Department of Physics, Chalmers University of Technology, Göteborg, Sweden. (Goldys, Ewa) || Department of Pharmacology & Neuroscience University of North Texas Health Science Center, Fort Worth, TX 76107. (Schetz, John) |
| Abstract | Summary form only given. Surface-assembled thin molecular layers and nanostructures offer unique opportunities to study processes and mechanisms of macromolecular (protein-protein or/and protein-ligand) interactions. Possibility to capture the conformational information buried in quantum-level interactions offers unique opportunities for the dynamic monitoring of biophysical and biochemical processes at the molecular level. In this report we are presenting novel developments for bioassays with enhanced sensitivity and capabilities due to quantum-photonic interactions of a fluorescent reporter with metallic nanostructures. We discus three cases of plasmons: (1) in nanometer size particles (localized plasmons and metal enhanced fluorescence); (2) in thin metallic film (surface plasmons and directional surface plasmon coupled emeission SPCE); and (3) combination of nanoparticles with continous nanosurfaces (enhanced and directional emission). We will present examples of enhanced immunoassays, background suppression, and applications of this novel technology to the simultaneous measurement of changes in conformation, orientation and mass that occur when biomolecular complexes form. |
| Starting Page | 240 |
| Ending Page | 240 |
| File Size | 743086 |
| Page Count | 1 |
| File Format | |
| ISBN | 0780397738 |
| DOI | 10.1109/METAMAT.2006.335048 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2006-10-16 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Proteins Cells (biology) Fluorescence Plasmons Genetics Biology Nanostructures Nanobioscience Biological cells Immune system |
| Content Type | Text |
| Resource Type | Article |
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