NDLI: Optimization of immunochemistry for sensing techniques to detect pesticide residues in water

Content Provider	IEEE Xplore Digital Library
Author	Uthuppu, B. Kostesha, N. Jakobsen, M.H. Kiersgaard, S.M. Aamand, J. Jorgensen, C.
Copyright Year	2011
Description	Author affiliation: Dept. of Environmental Riskassessment, DHI, Agern Alle 5, DK - 2970 Hørsholm, Denmark (Jorgensen, C.) \|\| Dept. of Geochemistry, Geological Survey of Denmark and Greenland (GEUS), Østervoldgade 10, DK-1350 Copenhagen K, Denmark (Kiersgaard, S.M.; Aamand, J.) \|\| Dept. of Micro-and Nanotechnology, Technical University of Denmark (DTU), DK - 2800 Kgs Lyngby, Denmark (Uthuppu, B.; Kostesha, N.; Jakobsen, M.H.)
Abstract	We are working on the development of a real-time electrochemical sensor based on an immunoassay detection system to detect and quantify the presence of pesticide residues in ground water. Highly selective and sensitive immuno-reactions are being investigated to be optimized in order to bring them into the level of real-time in-line sensors. In this project a competitive immunoassay between surface immobilized 2,6-dichlorobenzamide (BAM) haptens and BAM present in the water sample using an anti-BAM monoclonal antibody is being described. 2,6-Dichlorobenzamide (BAM) is a degradation product of the herbicide, dichlobenil which has been used extensively in the past and it is among the most frequently found pesticide residues in European ground water. BAM is highly resistant to further degradation and is fairly soluble in water. We have synthesized and immobilized a small library of BAM haptens and compared the affinity constants of the antibody towards this library. Furthermore, since regeneration of the BAM-hapten surface is a prerequisite for the development of a real-time electrochemical sensor with immunoassay-based detection, studies on regeneration of surfaces, modified with the newly synthesized BAM-haptens has been preformed and compared and correlated to the measured affinity constants. By using conventional ELISA we were able to indicate that one of the immobilized BAM haptens with an intermediate affinity towards the anti-BAM antibody was better in terms of regeneration. Design and fabrication of a fully automated microfluidic based on this immunoassay and electrochemical detection are in progress.
Starting Page	173
Ending Page	176
File Size	696812
Page Count	4
File Format	PDF
ISBN	9781424480630
e-ISBN	9781424480647
DOI	10.1109/SAS.2011.5739792
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-02-22
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Real time systems electrochemistry surface modification real-time sensor Substrates Degradation 2,6-Dichlorobenzamide (BAM) anti-BAM monoclonal antibody Libraries Water pollution BAM-haptens Microfluidics Immune system ELISA
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Protozoa graze on the 2,6-dichlorobenzamide (BAM)-degrading bacterium Aminobacter sp. MSH1 introduced into waterworks sand filters

The most-probable-number enumeration of dichlobenil and 2,6-dichlorobenzamide (BAM) degrading microbes in Finnish aquifers

Study of degradation intermediates formed during electrochemical oxidation of pesticide residue 2,6-dichlorobenzamide (BAM) at boron doped diamond (BDD) and platinum-iridium anodes.

C and N isotope fractionation during biodegradation of the pesticide metabolite 2,6-dichlorobenzamide (BAM): potential for environmental assessments.

Optimization of an immunoassay of 2,6-dichlorobenzamide (BAM) and development of regenerative surfaces by immunosorbent modification with newly synthesised BAM hapten library.

Dichlobenil and 2,6-dichlorobenzamide (BAM) dissipation in topsoil and deposits from groundwater environment within the boreal region in southern Finland

Notice of RetractionPreparation of monoclonal antibody against DDA and development of a sensitive ELISA for detection metabolilite of organochlorine pesticide DDT

Optimization of an immunoassay of 2,6-dichlorobenzamide (BAM) and development of regenerative surfaces by immunosorbent modification with newly synthesised BAM hapten library.

Rationally Designed Synthetic Haptens to Generate Anti-Ciguatoxin Monoclonal Antibodies, and Development of a Practical Sandwich ELISA to Detect Ciguatoxins

Optimization of immunochemistry for sensing techniques to detect pesticide residues in water

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