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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Yan Chen Xian-he Huang Hong Chen Hua-shan Shi |
| Copyright Year | 2013 |
| Description | Author affiliation: Guangan Vocational & Tech. Coll., Guangan, China (Hong Chen) || Sch. of Autom. Eng., Univ. of Electron. Sci. & Technol. of China, Chengdu, China (Yan Chen; Xian-he Huang) || State Key Lab. of Biotherapy, Sichuan Univ., Chengdu, China (Hua-shan Shi) |
| Abstract | The immobilization of cancer antigens onto the surface of piezoelectric immunosensor by a novel method was presented and evaluated for its potential use in early cancer detection. A 3rd overtone 10MHz AT-cut quartz crystal resonator was applied to detect the antibody of NuTu-19 ovarian cancer cell in serum samples using this novel immobilization method. The NuTu-19 ovarian cancer cells were planted on the electrode surface, the resulting very small mass change is then transformed into the change in frequency of crystal resonator. A self-made frequency counter was employed to display the frequency change. The change in resonance frequency was from 108 to 282Hz. The antibody content of NuTu-19 detected in immunized serum samples was 341ng/ul, and only 8% deviation compare to the data obtained from the protein quantification method. This immobilization method gave good results in terms of sensitivity, reproducibility and stability. |
| Sponsorship | IEEE Ultrason., Ferroelectr., Freq. Control Soc. |
| Starting Page | 1 |
| Ending Page | 3 |
| File Size | 194792 |
| Page Count | 3 |
| File Format | |
| ISBN | 9781479932894 |
| e-ISBN | 9781479932887 |
| DOI | 10.1109/SPAWDA.2013.6841138 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2013-10-25 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Proteins Piezoelectric immunosensor Quartz crystal Stability Resonant frequency Crystals Immobilization Cancer detection Educational institutions Ovarian cancer cell Immune system Cancer |
| Content Type | Text |
| Resource Type | Article |
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