NDLI: Three-Dimensional Reconstruction and Visualization of Xylem Vessels of Wheat Nodal Root

Content Provider	IEEE Xplore Digital Library
Author	Haiwen Wu Jaeger, M. Mao Wang Baoguo Li Bao Gui Zhang
Copyright Year	2009
Abstract	Descriptions of root internal structure are often limited to 2D cross-sections, with little information about three dimensions, and the evolution of xylem vessels along root axis has not been clearly presented. A computer-aided 3D-reconstrution method was developed based on paraffin serial slices techniques. The image processing protocol involved slice images alignment, registration, and 3D reconstruction. The stability of this approach was quantified and proven to be satisfactory. The 3D reconstruction conducted on wheat nodal roots illustrated that metaxylem vessel number decreased along root axis. Most vessels connected to the existent or new-formed vessels by lateral cell walls before disappearing. Thus metaxylem vessels in longitudinal profiles were not straightforward. The proposed imaging technique was low cost. It allowed various visualizations and quantitative comparisons of root structures. In the ongoing work, the technique is used to illustrate the spatial coordination of the radial pathway (passage cells, lateral roots), and longitudinal conduits (xylem vessels) of water and nutrient transport.
Starting Page	384
Ending Page	390
File Size	712020
Page Count	7
File Format	PDF
ISBN	9781769539881
e-ISBN	9781424463305
DOI	10.1109/PMA.2009.39
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-11-09
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Visualization Costs Protocols Evolution (biology) Image processing Microscopy Anatomical structure Soil Biology Image reconstruction
Content Type	Text
Resource Type	Article

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