NDLI: Preparation of an antibacterial peptide bovine lactoferricin by genetic engineering

Content Provider	IEEE Xplore Digital Library
Author	Qiang Deng Bate Zhuoping Ding Chengchu Liu Jianzhang Lu
Copyright Year	2010
Description	Author affiliation: Institute of Health Food, Zhejiang Academy of Medical Science, Hangzhou 310013, China (Jianzhang Lu) \|\| College of Food Science and Technology, Shanghai Ocean University, 201306, China (Qiang Deng; Bate; Zhuoping Ding; Chengchu Liu)
Abstract	Lactoferricin (Lfcin) is an amphipathic and cationic peptide with strong antibacterial activities against a broad range of Gram-positive, Gram-negative bacteria. It is usually generated by the pepsin-mediated digestion of lactoferrin. This paper investigated the potential for production of bovine lactoferricin (LfcinB) through genetic engineering. Three primers were designed according to amino acid sequence of bovine lactoferricin and then used to construct an oligonucleotide fragment (BeL) encoding lactoferricin. Genetically engineered bacteria (E. coli BL21-pEC- BeL) was successfully constructed after the BeL was inserted into a pEC vector and transformed into E. coli BL21 (DE3). Expression of the pEC vector in E. coli BL21-pEC-BeL was induced by addition of lactose and resulted in production of a fusion protein containing the target peptide LfcinB inside the E. coli cells. Purification of fusion protein was carried out by lysis of bacterial cells with lysozyme, followed by urea precipitation and ethanol precipitation method. The recombinant LfcinB was isolated by acid hydrolysis of the fusion protein to remove the fusion partner and purified through CM52 ion exchange column and Sephadex G-25 column. The molecular weight of the recombinant LfcinB was 3.4KD and its amino acid profile was consistent with the target peptide.
Starting Page	467
Ending Page	471
File Size	461581
Page Count	5
File Format	PDF
ISBN	9781424464951
e-ISBN	9781424464982
DOI	10.1109/BMEI.2010.5639241
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-10-16
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Microorganisms antibacterial peptide Peptides Bovine Escherichia coli fusion protein expression recombinant bovine lactoferricin Amino acids Anti-bacterial Encoding genetical engineering
Content Type	Text
Resource Type	Article

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