NDLI: Comparison of fluorophore and peroxidase labeled aptamer assays for MUC1 detection in cancer cells

Content Provider	IEEE Xplore Digital Library
Author	Flanagan, S. Limson, J. Fogel, R.
Copyright Year	2014
Description	Author affiliation: Biotechnol. Innovation Centre, Rhodes Univ., Grahamstown, South Africa (Flanagan, S.; Limson, J.; Fogel, R.)
Abstract	Aptamers hold great promise for cancer diagnosis and therapy. Several biosensors incorporate aptamers as biorecognition elements for tumor markers although few evaluate their detection in a native conformation and cellular micro-environment. In this study, fluorophore and peroxidase labeled aptamer configurations were compared for the detection of MCF7 breast and SW620 colon cancer cell lines expressing the tumor marker MUC1. Fluorescence based detection showed selective binding to the cell lines relative to a nonbinding control sequence with sequence specific binding differences between MUC1 aptamers accredited to variation in the glycosylation state of expressed MUC1. The peroxidase labeled assay showed high detection sensitivity although low binding specificity was observed for the MUC1 aptamers to the cell lines. Results suggest that aptamers susceptible to non specific binding to cells may limit the applicability of enzymatic amplification to improve aptasensor sensitivity.
Starting Page	292
Ending Page	295
File Size	933593
Page Count	4
File Format	PDF
ISBN	9781479923465
DOI	10.1109/BioCAS.2014.6981720
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-10-22
Publisher Place	Switzerland
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	enzyme linked oligonucleotide assay Dairy products Powders MCF7 Fluorescence biosensor platform SW620 MUC1 Cells (biology) fluorescence assay Aptamer Biosensors Cancer Tumors
Content Type	Text
Resource Type	Article

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