NDLI: A Proteome Characteristic Pattern of Unstable Angina was Found by Two-Dimensional Difference Gel Electrophoresis and Least Angle Regression

Content Provider	IEEE Xplore Digital Library
Author	Huihui Zhao Jianxin Chen Na Hou Weidong Lu Wei Wang
Copyright Year	2009
Abstract	Unstable angina is now a severe burden on society and family in both industrialized and developing countries, but the traditional related factors can not explain the whole situations and the traditional “golden indicator” for the diagnosis meet many new challenges. The aim of this study was to analyze the proteome characteristic pattern of unstable angina. Plasma samples were obtained from twelve unstable angina patients and twelve healthy volunteers. A polyclonal antibody affinity column was used to remove the six most abundant proteins. Then, the two classes of samples were separated by Two-dimensional difference gel electrophoresis (2D-DIGE). The differentially expressed protein spots were selected and identified with matrix-assisted laser desorption /ionization time-of-flight mass spectrometry (MALDI-TOF-MS) or MS-MS. In the end, using least angle regression algorithm, we studied the proteome characteristic pattern of unstable angina. The result is that, there are significant difference between unstable angina patients and healthy volunteers. The twenty-four proteins made pattern gain by least angle regression could distinguish unstable angina patients from the healthy people and it is probably the proteome characteristic pattern of unstable angina. To have a conclusion, the twenty-four proteins made pattern maybe the proteome characteristic pattern of unstable angina.
Starting Page	104
Ending Page	108
File Size	989522
Page Count	5
File Format	PDF
ISBN	9780769537368
DOI	10.1109/ICNC.2009.420
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-08-14
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Plasma applications proteomics data mining Electrokinetics Fluorescence Mass spectroscopy least angle regression Proteins Plasma properties Hospitals Proteomics characteristic pattern two-dimensional difference gel electrophoresis Plasma displays unstable angina plasma Medical diagnostic imaging
Content Type	Text
Resource Type	Article

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