NDLI: Surface-enhanced Raman-scattering biosensor on nanoparticle island substrates for DNA detection

Content Provider	IEEE Xplore Digital Library
Author	Yuan, W. Ho, H.P. Lee, R. Kong, S.K.
Copyright Year	2008
Description	Author affiliation: Dept. of Electron. Eng., Chinese Univ. of Hong Kong, Shatin, China (Yuan, W.; Ho, H.P.) \|\| Dept. of Biochem., Chinese Univ. of Hong Kong, Shatin, China (Lee, R.; Kong, S.K.)
Abstract	Summary form only given. This work presents a systematic study of the surface-enhanced Raman-scattering (SERS) properties of nanoparticle island substrates (NIS) and their application for oligonucleotide target detection. Using NIS that are structurally well characterized by atomic force microscopy (AFM) and UV-VIS extinction spectrum, it demonstrates the correlation of nanostructure of substrates and extinction maximum $(¿_{max})$ of substrates fabricated on different conditions, i.e. thickness of metal Ag. To effectively implement SERS on NIS and find out optimal condition for DNA detection, the relationship between extinction maximum $(¿_{max})$ and SERS enhancement factor (EF) was explored in detail. In fact, this work demonstrates high S/N ratio SERS spectra can be achieved for NIS with $¿_{max}$ within a spectrum window (~60 nm) consisting by the excitation wavelength (514 nm) and the scattered Raman wavelength, and highest EF measured is about 4 × $10^{8}$ with thickness of Ag being 50 A. Detection of oligonucleotide target has been performed with a sandwich assay scheme. In this scheme a layer of thiol modified receptor oligonucleotide was assembled on NIS and 3' of oligonucleotide probe has been labeled with a Raman-active dye. Furthermore, We compared the detection performance of strategies using probe oligonucleotide with or without gold nanoparticles (Au-NPs, 20 nm) capped on 5'. The experimental results reveal that the DNA detection implemented with NIS can provide high sensitivity, and both dynamic range and diction limit can be amplified with the aid of Au-NPs on 5' of probes. The current detection limits of NIS with and without Au-NPs are 0.4 femtomolar and 1 nanomolar respectively.
Starting Page	1
Ending Page	1
File Size	184457
Page Count	1
File Format	PDF
ISBN	9781557528636
DOI	10.1364/AOE.2008.SuH2
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2008-10-30
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	OSA
Subject Keyword	Performance evaluation Atomic force microscopy Wavelength measurement Raman scattering DNA Object detection Biosensors Probes Assembly Thickness measurement
Content Type	Text
Resource Type	Article

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