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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Shilun Feng Tao Dong |
| Copyright Year | 2014 |
| Description | Author affiliation: Dept. of Micro- & Nanosystem Technol., HOgskolen i Buskerud og Vestfold, Tonsberg, Norway (Shilun Feng; Tao Dong) |
| Abstract | A novel multifunctional two-sides silicon lab-on-chip (LOC) was designed and fabricated for providing a high-efficient long-term continuous analysis platform of measuring the concentration of adenosine 5'-triphosphate (ATP) based on bioluminescence. This lab-on-chip mainly consists of Y shape internal ribbed micro mixers, T-junction droplet generators, different kinds of spiral time delay channels (TD-Cs) and the chambers. Enzyme solutions and ATP were separately injected into the chip at different flow rates through 3 inlet ports. Due to the advantage of Segmented Flow Analysis (SFA), it was introduced and applied with the T-junction droplet generators in this paper, and thus oxygen was injected into the central of the bottom surface through another main inlet periodically. Numerical investigations prove that combing with SFA and T-junction droplet generator is stable to transport the sample; micro mixer can achieve an outstanding mixing efficiency in a small space; the detection of ATP standard solution at optimal conditions was successful, keeping a good linearity between the concentration with the intensity of light during the range from $10^{-5}$ mol /ml to $10^{-9}$ mol/ml. |
| Sponsorship | IEEE Instrum. Meas. Soc. |
| Starting Page | 1 |
| Ending Page | 4 |
| File Size | 918765 |
| Page Count | 4 |
| File Format | |
| e-ISBN | 9781479929214 |
| DOI | 10.1109/MeMeA.2014.6860076 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2014-06-11 |
| Publisher Place | Portugal |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Mixers Generators Shape Biochemistry Microfluidics Liquids Semiconductor device measurement adenosine 5'-triphosphate (ATP) lab-on-chip internal ribbed micro mixer T-junction droplet generator |
| Content Type | Text |
| Resource Type | Article |
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