NDLI: Digital microfluidic biochip design for protein crystallization

Content Provider	IEEE Xplore Digital Library
Author	Tao Xu Thwar, P. Srinivasan, V. Pamula, V.K. Chakrabarty, K.
Copyright Year	2007
Description	Author affiliation: Duke Univ., Durham (Tao Xu)
Abstract	Proteins crystallization is a commonly used technique for protein analysis. It predicts the three-dimensional (3D) arrangement of the constituent amino acids, which indicates the specific biological function of a protein. Protein crystallization experiments are typically carried out in well-plates; as a result, these experiments are slow, expensive, and errors are likely due to the need for repeated human intervention. Recently, droplet-based "digital" microfluidics has been used for executing protein assays on a chip. Protein samples are enclosed in droplets, which are manipulated using the principle of electrowetting-on-dielectric. Digital microfluidics offers a high degree of automation, and it significantly reduces the volumes of proteins required for crystallization. We present the design of a multi-well plate microfluidic biochip for protein crystallization, which can transfer protein samples, prepare candidate solutions, and carry out crystallization automatically. To reduce the manufacturing cost of such devices, we present an efficient algorithm to generate a pin-assignment plan for the proposed design. The resulting biochip enables control of a large number of on-chip electrodes using only a small number of pins.
Starting Page	140
Ending Page	143
File Size	145171
Page Count	4
File Format	PDF
ISBN	9781424418121
DOI	10.1109/LSSA.2007.4400904
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-11-08
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Microfluidics Proteins Crystallization Amino acids Humans Manufacturing automation Costs Algorithm design and analysis Automatic control Electrodes
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in