NDLI: Regulating the mechanical properties of cells using a non-UV light-addressable hydrogel patterning process

Content Provider	IEEE Xplore Digital Library
Author	Na Liu Changlin Zhang Lianqing Liu Yuechao Wang Gwo-Bin Lee Li, W.J.
Copyright Year	2014
Description	Author affiliation: Dept. of Mech. & Biomed. Eng., City Univ. of Hong Kong, Hong Kong, China (Li, W.J.) \|\| Dept. of Power Mech. Eng., Nat. Tsinghua Univ., Hsinchu, Taiwan (Gwo-Bin Lee) \|\| State Key Lab. of Robot., Shenyang Inst. of Autom., Shenyang, China (Na Liu; Changlin Zhang; Lianqing Liu; Yuechao Wang)
Abstract	The determination of the mechanical properties of cells plays an important role in biological studies and has gained acceptance recently as a possible label-free biomarker for cell status determination or diseases detection. Investigations on how external cellular properties affect cell mechanics are helpful in understanding cell disease processes and cell morphogenesis, which are of large significance in medical science. Although most researchers have focused on individual cell mechanics, or the effect of substrate stiffness on cells, cell mechanical response due to interactions among cells is yet to be examined. A reason for this is that the study of cell mechanical response to cell shape requires one to use a cell patterning process. However, existing cell patterning methods are very complex and time-consuming. In this paper, we describe a practical and rapid technique that can easily pattern cells into desired shapes, which allows investigations of the effect of external environment on cell stiffness. In the new technique, Poly-(ethylene) glycol diacrylate (PEGDA) hydrogel film with thickness 70-100 nm is controllably patterned on a hydrogenated amorphous silicon (a-Si:H) substrate by polymerizing PEGDA molecules in-situ using programmable visual light patterns. The idea is to enable the confinement of cells cultured on the hydrogels into special areas. The elastic modulus of the patterned cells is measured using an atomic force microscope. Experimental results have demonstrated the versatility of the technique as a tool for cell pattering and exploration of cell mechanics under external mechanical stimuli.
Starting Page	5866
Ending Page	5870
File Size	587011
Page Count	5
File Format	PDF
ISBN	9781479936854
DOI	10.1109/ICRA.2014.6907722
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-05-31
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Substrates Shape Polymers Force Films Cells (biology)
Content Type	Text
Resource Type	Article

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