NDLI: Active contours for cell tracking

Content Provider	IEEE Xplore Digital Library
Author	Ray, N. Acton, S.T.
Copyright Year	2002
Description	Author affiliation: Dept. of Electr. & Comput. Eng., Virginia Univ., Charlottesville, VA, USA (Ray, N.)
Abstract	This paper introduces an active contour or snake-based method for tracking cells within a video sequence. Specifically, we apply our cell tracking techniques to rolling leukocytes observed in vivo (in living animal) from video microscopy. The analysis of leukocyte motion reveals cues about the mechanism of inflammatory disease. To attack the problem of tracking leukocytes in vivo, the proposed snake tracker utilizes shape and size information specific to the leukocytes. The principal contribution of this work lies in introducing the shape and size constraint as a geometric primitive in the parametric snake energy model. The energy functional is then minimized through the basic principles of the calculus of variations to obtain the Euler equations used in contour updating. We have developed a partial differential equation (PDE) based generalized gradient vector flow (GVF) that accommodates for contrast changes and weak cell edges. Whereas previous GVF models are sensitive to initial contour placement, the modified GVF construction with Dirichlet type boundary condition (BC) allows a snake tracker to be robust for a wide range of initial positions. Another contribution in this work is to incorporate an energy term in the snake model that eliminates the need for explicitly resampling the snake contour intermittently as performed in traditional snake evolution. Using animal experiments, we compare the accuracy of the proposed snake tracker with the correlation and centroid based tracker and show that the proposed tracker is superior in terms of increased number of frames tracked and reduced localization error.
Sponsorship	IEEE Comput. Soc.
Starting Page	274
Ending Page	278
File Size	273865
Page Count	5
File Format	PDF
ISBN	0769515371
DOI	10.1109/IAI.2002.999932
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-04-07
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Active contours White blood cells In vivo Animals Shape Video sequences Microscopy Motion analysis Diseases Solid modeling
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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