NDLI: Single cell assay on cd-like lab chip using centrifugal single cell trap

Content Provider	IEEE Xplore Digital Library
Author	Sung-Woo Lee In-Hye Lee Sung-Shin Ryu Seung-Min Kwak Kyeong-Sik Shin Ji-Yoon Kang Hyo-Il Jung Tae-Song Kim
Copyright Year	2007
Description	Author affiliation: Korea Inst. of Sci. & Technol., Seoul (Sung-Woo Lee; In-Hye Lee; Sung-Shin Ryu; Seung-Min Kwak; Kyeong-Sik Shin; Ji-Yoon Kang)
Abstract	This paper reports a single cell assay in compact disc (CD)-like lab chip with trapped single cells by centrifugal force. The main propose of this study was realization of cell assay on chip with the advantage of excluding the paracrine effect of adjacent cells. The channel in CD-like lab chip has many pitfalls at the outer side, in which cells were trapped respectively. The trap was able to fix each cell and the chemicals were treated by centrifugal pumping. Parameters that affects the trapping of a cell were the size of cells, the structure of micro-pattern, the magnitude of centrifugal force and the duration time. The trap ratio of cells was around 80% at optimal condition. Cytotoxicity and apoptosis test was done in CD and it was compared with conventional method to see the effect of single cell assay. Cytotoxicity was tested by the treatment of parformaldehyde and the apoptosis test was done by illumination UV light. The results of both assays indicated the overall trend of single assay in chip and conventional assay was same. However, apoptosis assay showed that the survival rate of single cell assay in chip was higher than that of conventional assay. Although it is still unclear that the result was caused by the effect of crowded cells, this single cell assay platform could be one of valuable tools in single cell assay.
Starting Page	557
Ending Page	560
File Size	592500
Page Count	4
File Format	PDF
ISBN	9781424409501
ISSN	10846999
DOI	10.1109/MEMSYS.2007.4433158
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-01-21
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Testing Microfluidics Valves Chemicals Proteins Monitoring Mechanical engineering Telephony Lighting Cause effect analysis
Content Type	Text
Resource Type	Article
Subject	Condensed Matter Physics Electronic, Optical and Magnetic Materials Mechanical Engineering Electrical and Electronic Engineering

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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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