NDLI: Adaptive silver films for bio-array applications

Content Provider	IEEE Xplore Digital Library
Author	Drachev, V.P. Nashine, V. Thoreson, M.D. Khaliullin, E.N. Ben-Amotz, D. Davisson, V.J. Shalaev, V.M.
Copyright Year	2004
Description	Author affiliation: Sch. of Electr. & Comput. Eng., Purdue Univ., West Lafayette, IN, USA (Drachev, V.P.)
Abstract	Summary form only given. The adaptive property of e-beam evaporated silver films has been studied and employed for protein sensing with surface-enhanced Raman scattering (SERS). Our approach for sensing proteins is based on adaptive silver films (ASFs); the method allows fine nanostructure rearrangement under protein deposition that makes it possible to soft-adsorb proteins on the metal surface and to form aggregates of nanoparticles which are optimal for SERS. We demonstrate that adaptive nanostructured films can be used for the observation of differences in SERS spectra of recombinant human insulin and insulin lispro at sub-monolayer protein surface densities and provide macroscopic enhancement factor of about 3/spl times/10/sup 6/ . Since human insulin and its analog have the same set of side chains and differ only in conformational states, the observed difference indicates the preservation of the conformational state with the use of adaptive metal substrates. Direct, label-free detection of antibody-antigen binding at a monolayer protein concentration has been also demonstrated, using SERS detection on adaptive silver films. This includes evidence of distinct SERS spectral changes upon antigen-antibody binding and independent biochemical sandwich assay validation studies, which confirm that antibodies retain binding properties on ASFs. It is shown that proteins stabilize the ASFs and restructure them so that proteins can preserve their binding activity. We demonstrate the ability to employ various detection methods with one substrate, including label-free SERS, chemiluminescence, fluorescence, and further analysis of protein spot retrieved from the surface.
File Size	37967
File Format	PDF
ISBN	0780385578
DOI	10.1109/LEOS.2004.1363298
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2004-11-11
Publisher Place	Puerto Rico
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Silver Insulin Chemistry Protein engineering Humans Application software Biomedical engineering Raman scattering Aggregates Nanoparticles
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in