NDLI: Micro fluidic device to control the position and to analyze the condition of C. elegans as a bioindicator

Content Provider	IEEE Xplore Digital Library
Author	Jaehoon Jung Nakajima, M. Kojima, M. Tajima, H. Fukuda, T.
Copyright Year	2012
Description	Author affiliation: Center for Micro-nano Mechatronics, Nagoya University, 464-8603, Japan (Nakajima, M.) \|\| Department of Micro-nano Systems Engineering, Nagoya University, 464-8603, Japan (Jaehoon Jung; Kojima, M.; Tajima, H.; Fukuda, T.)
Abstract	We proposed a micro fluidic device to control the position of Caenorhabditis elegans (C. elegans) and to analyze the condition of C. elegans as a bioindicator during its lifespan. In this device, we can filter C. elegans depending on size and control the position of C. elegans to culture and test C. elegans and to measure the capacitance change. The capacitance change depends on the body volume of C. elegans. Through the correlation with the capacitance change and the body volume of C. elegans, the body volume of C. elegans is assessed without imaging system. The proposed micro fluidic device was implemented using a PDMS layer and a glass substrate that were bonded together. The PDMS layer had a valve system and pattern rows to filter, culture and test C. elegans. A pair of electrodes is used to measure the capacitance change. In this study, we filtered C. elegans into the same size by filter and one C. elegans was introduced into a chamber row to be cultured and tested. And it was confirmed the correlation between the capacitance change and the body volume of C. elegans. And the proposed micro fluidic device will be applied to observe the effect of chemical or toxicant like heavy metal (Cd) on C. elegans as bioindicator.
Starting Page	932
Ending Page	937
File Size	1480891
Page Count	6
File Format	PDF
ISBN	9781467317375
ISSN	21530858
e-ISBN	9781467317368
DOI	10.1109/IROS.2012.6385995
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2012-10-07
Publisher Place	Portugal
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrodes Capacitance Microfluidics Fluidic microsystems Cadmium Grippers Glass
Content Type	Text
Resource Type	Article
Subject	Control and Systems Engineering Computer Science Applications Computer Vision and Pattern Recognition Software

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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