NDLI: Intracellular calcium release during in vitro mammalian embryonic development in the presence of Kalanchoe pinnata extract

Content Provider	IEEE Xplore Digital Library
Author	Nor Azila Md Ludin Farida Zuraina Mohd Yusof Hashim, Nooraain
Copyright Year	2011
Description	Author affiliation: Faculty of Applied Sciences, Universiti Teknologi Mara (UiTM), 40450 Shah Alam, Selangor, MALAYSIA (Nor Azila Md Ludin; Farida Zuraina Mohd Yusof; Hashim, Nooraain)
Abstract	The study was conducted to investigate the potential of using herbal extract which was Kalanchoe pinnata (Setawar) as supplement into the culture medium for in vitro embryonic development specifically during early cleavage stages of rabbit and mouse embryonic developments. In addition, calcium releases during each of the stages were detected and compared with those cultured in Whitten Medium (WM) which were the control medium. Two concentrations of the extract designated as T1 (1 mg/ml) and T2 (2.5 mg/ml) were used. The 8-cell stage was recorded among rabbit embryos cultured in K. pinnata supplemented medium with the percentage of 15.15% of development found in 1 mg/ml of the extract. Developmental stages of the 1-cell mouse embryos halted at 4-cell stage, in both 1 mg/ml and 2.5 mg/ml extract. Meanwhile, by using 2-cell embryos, the developmental stage had reached to morula in WM medium supplemented with 2.5 mg/ml of the extract. The later also showed the highest luminescence signals for calcium release during cleavage development.
Starting Page	897
Ending Page	901
File Size	497218
Page Count	5
File Format	PDF
ISBN	9781467300216
e-ISBN	9781467300209
DOI	10.1109/CHUSER.2011.6163867
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-12-05
Publisher Place	Malaysia
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Rabbits Embryo Kalanchoe pinnata Calcium Intracellular calcium Luminescence Materials Mice Embryonic development in vitro In vitro
Content Type	Text
Resource Type	Article

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