NDLI: Automated segmentation of cell structure in microscopy images

Content Provider	IEEE Xplore Digital Library
Author	Kerrison, Nicole Bulpitt, Andy
Copyright Year	2014
Description	Author affiliation: School of Computing, University of Leeds, Woodhouse Lane, LS2 9JT, U.K. (Kerrison, Nicole; Bulpitt, Andy)
Abstract	Understanding cell movement is important in helping to prevent and cure damage and disease. Increasingly, this study is performed by obtaining video footage of cells in vitro. However, as the number of images obtained for cellular analysis increases, so does the need for automated segmentation of these images, since this is difficult and time consuming to perform manually. We propose to automate the process of segmenting all parts of a cell visible in DIC microscopy video frames by providing an efficient method for correcting the lighting bias and a novel combination of techniques to detect different cell areas and isolate parts of the cell vital to their movement. To the best of our knowledge we contribute the only method able to automatically detect the thin cellular membranes in DIC images. We show that the method can be used to isolate features in order to detect variations vital to motility in differently affected cells.
Starting Page	98
Ending Page	105
File Size	1313213
Page Count	8
File Format	PDF
ISBN	9789897581335
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-01-05
Publisher Place	Portugal
Access Restriction	Subscribed
Rights Holder	SCITEPRESS
Subject Keyword	Image segmentation Shape Image color analysis Image edge detection Microscopy Segmentation Lamellipodia Lighting Cell Motility Lighting Correction DIC
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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