NDLI: Optimization of electrofusion in vitro for rhabdomyosarcoma cells

Content Provider	IEEE Xplore Digital Library
Author	Yeo, C.X.F. Ying Ying Woh Kham Lim, C.S.D. Kian Hwa Tan Eng Lee Tan
Copyright Year	2011
Description	Author affiliation: School of Mechanical and Aerospace Engineering, Nanyang Technological University, Singapore (Yeo, C.X.F.; Ying Ying Woh Kham; Lim, C.S.D.) \|\| Centre for Biomedical and Life Sciences, Singapore Polytechnic, Singapore (Kian Hwa Tan; Eng Lee Tan)
Abstract	Cell-cell fusion is an important natural and engineered process for in-depth studies into hybridomas, developmental biology, immunology and various cellular therapies. It is also a powerful tool for analysis of gene expression, chromosomal mapping, antibody production, cloning mammals, and cancer immunotherapy. However, research so far has primarily focused on cell models such as C.elegans, drosophila, myoblasts, spleen-myeloma cell hybrids and various plant protoplasts. Rhabdomyosarcoma cells are a rare form of musculoskeletal cancer cells found in the head, neck, and other less skeletal areas of the human cancer patient's body. As these cells do not normally undergo fusion naturally, they are an interesting model for studying cell fusion. Among all the techniques for fusion, electrofusion (electroporation) can be applied to a wide range of cell types with high efficiency and high post-fusion viability. By coupling these cells with this technique, the effects on cell proliferation, growth pattern, and hybridoma count were investigated. Overall, the experimental results showed that an adequate electrical stimulation helped to facilitate the fusion and proliferation of the RD cells. Furthermore, a DC current produced the highest number of hybridomas, while maintaining the highest proliferation rate. After subtracting for the control samples, an average fusion yield of 24% was obtained under this DC setting, which is comparable to the fusion yield of 20% obtained using the same technique by other researchers. This is a promising result for its application in the production of monoclonal antibodies for cancer research and treatment. This proof-of-concept is predicted to be useful to both the medical scientists in the field of immunology and cell-based therapy, as well as defense scientists working on chemical and biological warfare.
Starting Page	1
Ending Page	4
File Size	747117
Page Count	4
File Format	PDF
ISBN	9781424492763
e-ISBN	9781424492770
DOI	10.1109/DSR.2011.6026864
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-03
Publisher Place	Singapore
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Electrofusion Medical treatment Humans Rhabdomyosarcoma Hybdridoma Electroporation Cell Fusion Efficiency Cells (biology) Production Cell-cell Lab-on-a-chip Biomembranes Yield Cancer Immune system Monoclonal Antibody Production
Content Type	Text
Resource Type	Article

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J Membrane Biol (2012) 245:583–590 DOI 10.1007/s00232-012-9470-2 The Systematic Study of the Electroporation and Electrofusion of B16-F1 and CHO Cells in Isotonic and Hypotonic Buffer

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