NDLI: Identification of citrinin biosynthesis gene under the ultrasonic induction of Monascus purpureus

Content Provider	IEEE Xplore Digital Library
Author	Suktham, K. Suwansungsa, O. Wonganu, B. Kongruang, S. Roytrakul, S.
Copyright Year	2010
Description	Author affiliation: BIOTEC Central Research Laboratory, Thailand Science, Park, Pathumthani, Thailand (Roytrakul, S.) \|\| Department of Biotechnology, Faculty of Applied Science, King Mongkut's University of Technology North, Bangkok, Bangkok, Thailand (Suktham, K.; Suwansungsa, O.; Wonganu, B.; Kongruang, S.)
Abstract	Polyketide synthases are multifunctional enzymes that involve the synthesis of pigments and mycotoxin which is normally known as citrinin. The gene involving citrinin biosynthesis especially, acyltransferase in polyketide synthase gene of M. purpureus TISTR 3541 isolated in Thailand as the wild type and the ultrasonic induced mutation of this wild type as the mutants (generations 1–4, G1–G4) was investigated. The transcription level of the acyltransferase in M. purpureus TISTR 3541 wild type (G0) and of mutants (G1 to G4) was studied by using RT-PCR with the actin gene as a positive control. The nucleotide sequences were analyzed by using Automated DNA Sequencing. Results showed that the nucleotide sequences of acyltransferase in polyketide synthase gene of M. purpureus mutants and wild type were absolutely similar. When the sequence comparison was aligned, the obtained nucleotide sequence showed 100% sequence similarity with pksCT gene that involving in citrinin biosynthesis in M. purpureus. The RT – PCR results were in agreement with real – time PCR analysis which was showed that shape intact DNA band of M. purpureus TISTR 3541 G2 more than other generations resulting in the better acyltransferase expression than others.
Starting Page	35
Ending Page	39
File Size	224636
Page Count	5
File Format	PDF
ISBN	9781424477654
e-ISBN	9781424477661
DOI	10.1109/ICCCENG.2010.5560355
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-08-01
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Multiplexing Monascus purpureus Polyketide synthase Chemistry USA Councils DNA Production Pigments Acoustics Acyltransferease DNA sequencing
Content Type	Text
Resource Type	Article

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