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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Hell, S.W. |
| Copyright Year | 2015 |
| Description | Author affiliation: Max Planck Inst. for Biophys. Chem., Gottingen, Germany (Hell, S.W.) |
| Abstract | Summary form only given. For more than a century, it has been widely accepted that diffraction of light precludes any lens-based optical microscope from discerning details smaller than about half of the wavelength of light (~200 nm). However, in the 1990's we discovered that basic state transitions in a fluorophore can be exploited to eliminate the resolution-limiting role of diffraction. Since then, fluorescence microscopes have been developed that are now able to resolve on the nanometer scale. We discuss the basic principles of these nanoscopy (superresolution) concepts with particular emphasis on the first viable far-field `nanoscopy' method, STED microscopy. We show their scope of applications in the life sciences and beyond. |
| Starting Page | 1 |
| Ending Page | 1 |
| File Size | 60188 |
| Page Count | 1 |
| File Format | |
| ISBN | 9781557529688 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2015-05-10 |
| Publisher Place | USA |
| Access Restriction | Subscribed |
| Rights Holder | OSA |
| Subject Keyword | Optical diffraction Optical microscopy Biomedical optical imaging Chemistry Diffraction Microscopy Fluorescence |
| Content Type | Text |
| Resource Type | Article |
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