NDLI: A 48-well microfluidic platform based on immuno-NASBA assay to quantify traces of soluble biomarkers in water environment

Content Provider	IEEE Xplore Digital Library
Author	Xinyan Zhao Tao Dong Zhaochu Yang Karlsen, F. Egeland, E.B. Pires, N.M.M.
Copyright Year	2011
Description	Author affiliation: Norwegian Center of Expertise on Micro- & Nanotechnol., Vestfold Univ. Coll., Tønsberg, Norway (Xinyan Zhao; Tao Dong; Zhaochu Yang; Karlsen, F.; Egeland, E.B.; Pires, N.M.M.)
Abstract	Microfluidic devices can automatically perform multiple laboratory functions on a single, compact and integrated chip. This study demonstrates that a new concept lab-on-a-chip system is able to quantify soluble biomarkers in water environment with femtomolar sensitivity, high specificity and ability for middle throughput monitoring. This system is based on a new assay named `real-time immuno-nucleic acid sequence-based amplification' (immuno-NASBA), which general scheme is similar to a sandwich antibody-mediated immuno-PCR assay. However, the barcode DNA linked with antibodies in the immunoassay contains a T7 RNA polymerase promoter is utilized as the template in the following nucleic acid sequence-based amplification (NASBA) assay. Compared with PCR, NASBA is an isothermal amplification assay which can greatly simplify microfluidic devices with the similar ultra-sensitivity. Thanks to the simplification of NASBA chip, the prototype microfluidic device introduced in this paper contains 48 reaction chambers that can be used to measure the concentrations of multiple biomarkers at the same time. The immuno-NASBA chip provides a novel tool of water environmental protection to monitor water quality in both lakes and rivers, which can also be used to detect water-borne disease or early warning of water environment pollution.
Starting Page	1586
Ending Page	1589
File Size	499488
Page Count	4
File Format	PDF
ISBN	9781612843391
e-ISBN	9781612843407
DOI	10.1109/ISWREP.2011.5893334
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-05-20
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Protocols Biomarker Immuno-NASBA Immunity testing Water environment DNA Biomarkers Water pollution Ultra-sensitive assay Microfluidics Lab-on-chips Immune system Quantitative analysis
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ultra-sensitive microfluidic system based on IMRAMP assay to quantify hormones in blood

2nd Generation of Immuno-NASBA LOC Devices for Monitoring Biomarkers in Aquatic Environment

Ultra-Sensitive Immuno-Sensing Platform Based on Gold-Coated Interdigitated Electrodes for the Detection of Parathion

The biomarker pipeline: Novel microfluidic instrumentation for advancing proteomic discovery to clinical diagnostics

Usage of microfluidic lab-on-chips in biomedicine

Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

1196-Chamber compatible LOC device with bubble valves for signaling molecular profiling in blood

Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform

Accurate quantification of multiple biomarkers using microfluidic determination of tumor-specific antigenic sites in cancer tissues

A 48-well microfluidic platform based on immuno-NASBA assay to quantify traces of soluble biomarkers in water environment

Similar Documents

Ultra-sensitive microfluidic system based on IMRAMP assay to quantify hormones in blood

2nd Generation of Immuno-NASBA LOC Devices for Monitoring Biomarkers in Aquatic Environment

Ultra-Sensitive Immuno-Sensing Platform Based on Gold-Coated Interdigitated Electrodes for the Detection of Parathion

The biomarker pipeline: Novel microfluidic instrumentation for advancing proteomic discovery to clinical diagnostics

Usage of microfluidic lab-on-chips in biomedicine

Analysis and experiment of centrifugal force for microfluidic ELISA CD platform

1196-Chamber compatible LOC device with bubble valves for signaling molecular profiling in blood

Biosensing on the Centrifugal Microfluidic Lab-on-a-Disc Platform

Accurate quantification of multiple biomarkers using microfluidic determination of tumor-specific antigenic sites in cancer tissues

A 48-well microfluidic platform based on immuno-NASBA assay to quantify traces of soluble biomarkers in water environment