NDLI: A combined experimental and computational analysis of membrane potential variations in excitable cells in response to DC electric fields

Content Provider	IEEE Xplore Digital Library
Author	Hassan, N. Chatterjee, I. Publicover, N.G. Craviso, G.L.
Copyright Year	2002
Description	Author affiliation: Dept. of Electr. Eng., Nevada Univ., Reno, NV, USA (Hassan, N.)
Abstract	Two approaches were used to gain an understanding of the spatial variations in membrane potential resulting from exposure of cultured adrenal medullary chromaffin cells to a 20 V/cm DC electric field of different orientations. The finite element method was used to solve Poisson's equation for the potential in a Petri dish containing isolated spherical chromaffin cells or chromaffin cell aggregates of various configurations and the results of the computations were compared to the results obtained experimentally. The latter used fluorescence imaging of chromaffin cell membranes stained with the fluorescent voltage-sensitive dye di-8-ANEPPS to monitor variations in membrane potential. For single cells, the numerical results for membrane potential variation were in good agreement with the experimental results. For realistic chains of two or three cells, the results from both methods were in agreement only when the long axis of the chain was perpendicular to the electric field. For three cells forming a realistic, irregularly shaped aggregate, variations in membrane potential determined experimentally were more complex than those predicted by the numerical computations for regions of cell contact. The results provide a foundation for understanding how excitable cells respond to DC electric fields and identify parameters that influence these responses.
Starting Page	91
Ending Page	94
File Size	267802
Page Count	4
File Format	PDF
ISBN	0780375025
DOI	10.1109/CEIDP.2002.1048744
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2002-10-20
Publisher Place	Mexico
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Biomembranes Cells (biology) Aggregates Finite element methods Fluorescence Poisson equations Voltage Geometry Shape Solid modeling
Content Type	Text
Resource Type	Article

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