NDLI: Automated Analysis of Fluorescence Lifetime Imaging Microscopy (FLIM) Data Based on the Laguerre Deconvolution Method

Content Provider	IEEE Xplore Digital Library
Author	Pande, P. Jo, J.A.
Copyright Year	1964
Abstract	In fluorescence lifetime imaging microscopy (FLIM), fluorescence time decay at each pixel of the imaged sample are measured. Every recorded fluorescence decay corresponds to the time convolution of the instrument response with the intrinsic fluorescence impulse response function (IRF), from which the sample fluorescence lifetime is determined. To estimate the IRF, the instrument response thus needs to be deconvolved from the recorded fluorescence decay. We have recently introduced a novel FLIM time-deconvolution method based on the linear expansion of the fluorescence decays on an orthonormal Laguerre basis. Since this method allows simultaneous estimation of the IRFs at all pixels, it performs at least two orders of magnitude faster than standard algorithms. In its original implementation, however, the Laguerre basis, determined by the Laguerre parameter , is selected using a heuristic approach. Here, we present an automated implementation, whereby the Laguerre parameter is treated as a free parameter within a nonlinear least squares optimization scheme. The new implementation combines the unmatched inherent computational speed of the Laguerre deconvolution method with a systematic model selection approach. This method will thus facilitate applications of FLIM requiring automatic estimation of the spatial distribution of fluorescence lifetimes, such as in in vivo tissue FLIM imaging.
Sponsorship	IEEE Engineering in Medicine and Biology Society
Page Count	10
File Size	661877
Starting Page	172
Ending Page	181
File Format	PDF
ISSN	00189294
Volume Number	58
Issue Number	1
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-01-01
Publisher Place	U.S.A.
Access Restriction	One Nation One Subscription (ONOS)
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Pixel Deconvolution Instruments Imaging Estimation Signal to noise ratio time deconvolution Fluorescence lifetime imaging microscopy (FLIM) in vivo imaging Laguerre expansion of the kernel technique
Content Type	Text
Resource Type	Article
Subject	Biomedical Engineering

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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